Evidence map›Paper›PMID 35003840›Full record

ArticleBiomedical optics express2021

Simultaneous scattering compensation at multiple points in multi-photon microscopy.

Molly A May, Kai K Kummer, Marie-Luise Edenhofer, Jeiny Luna Choconta, Michaela Kress, Monika Ritsch-Marte, Alexander Jesacher

Open access · goldAbstract read
In one paragraph

Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Fast wavefront shaping for two-photon brain imaging with multipatch correction.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  5. Adaptive optics for optical microscopy [Invited].Biomedical optics express · 2023
    Review
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Molly A MayInstitute of Biomedical Physics, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria.
Kai K KummerInstitute of Physiology, Medical University of Innsbruck, Schöpfstraße 41, 6020 Innsbruck, Austria.
Marie-Luise EdenhoferInstitute of Physiology, Medical University of Innsbruck, Schöpfstraße 41, 6020 Innsbruck, Austria.
Jeiny Luna ChocontaInstitute of Physiology, Medical University of Innsbruck, Schöpfstraße 41, 6020 Innsbruck, Austria.
Michaela KressInstitute of Physiology, Medical University of Innsbruck, Schöpfstraße 41, 6020 Innsbruck, Austria.
Monika Ritsch-MarteInstitute of Biomedical Physics, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria.
Alexander JesacherInstitute of Biomedical Physics, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria.ORCID https://orcid.org/0000-0003-4285-9406
Innsbruck Medical University · AT

Funding

Austrian Science Fund FWF P 32146
6 · The paper itself

Abstract

The two-photon fluorescence imaging depth has been significantly improved in recent years by compensating for tissue scattering with wavefront correction. However, in most approaches the wavefront corrections are valid only over a small sample region on the order of 1 to 10 µm. In samples where most scattering structures are confined to a single plane, sample conjugate correction geometries can increase the observable field to a few tens of µm. Here, we apply a recently introduced fast converging scheme for sensor-less scattering correction termed "Dynamic Adaptive Scattering compensation Holography" (DASH) in a sample conjugate configuration with a high pixel count nematic liquid crystal spatial light modulator (LC-SLM). Using a large SLM allows us to simultaneously correct for scattering at multiple field points, which can be distributed over the entire field of view provided by the objective lens. Despite the comparably slow refresh time of LC-SLMs, we achieve correction times on the order of 10 s per field point, which we show is sufficiently fast to counteract scattering at multiple sites in living mouse hippocampal tissue slices.

Identifiers

PMID35003840
PMCPMC8713664
OpenAlexW3205845440

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.