Evidence map›Paper›PMID 35003074›Full record

ArticleFrontiers in immunology2021

Intrinsic Strand-Incision Activity of Human UNG: Implications for Nick Generation in Immunoglobulin Gene Diversification.

Marina Alexeeva, Marivi Nabong Moen, Xiang Ming Xu, Anette Rasmussen, Ingar Leiros, Finn Kirpekar, Arne Klungland, Lene Alsøe, Hilde Nilsen, Svein Bjelland

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Marina AlexeevaDepartment of Chemistry, Bioscience and Environmental Technology, University of Stavanger, Stavanger, Norway.
Marivi Nabong MoenDepartment of Chemistry, Bioscience and Environmental Technology, University of Stavanger, Stavanger, Norway.
Xiang Ming XuDepartment of Chemistry, Bioscience and Environmental Technology, University of Stavanger, Stavanger, Norway.
Anette RasmussenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Ingar LeirosDepartment of Chemistry, University of Tromsø-The Arctic University of Norway, Tromsø, Norway.
Finn KirpekarDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Arne KlunglandDepartment of Microbiology, Oslo University Hospital, Oslo, Norway.
Lene AlsøeDepartment of Clinical Molecular Biology, University of Oslo, Oslo, Norway.
Hilde NilsenDepartment of Clinical Molecular Biology, University of Oslo, Oslo, Norway.
Svein BjellandDepartment of Chemistry, Bioscience and Environmental Technology, University of Stavanger, Stavanger, Norway.
University of Stavanger · NOUniversity of Southern Denmark · DKAkershus University Hospital · NOOslo University Hospital · NOUiT The Arctic University of Norway · NOUniversity of Oslo · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Uracil arises in cellular DNA by cytosine (C) deamination and erroneous replicative incorporation of deoxyuridine monophosphate opposite adenine. The former generates C → thymine transition mutations if uracil is not removed by uracil-DNA glycosylase (UDG) and replaced by C by the base excision repair (BER) pathway. The primary human UDG is hUNG. During immunoglobulin gene diversification in activated B cells, targeted cytosine deamination by activation-induced cytidine deaminase followed by uracil excision by hUNG is important for class switch recombination (CSR) and somatic hypermutation by providing the substrate for DNA double-strand breaks and mutagenesis, respectively. However, considerable uncertainty remains regarding the mechanisms leading to DNA incision following uracil excision: based on the general BER scheme, apurinic/apyrimidinic (AP) endonuclease (APE1 and/or APE2) is believed to generate the strand break by incising the AP site generated by hUNG. We report here that hUNG may incise the DNA backbone subsequent to uracil excision resulting in a 3´-α,β-unsaturated aldehyde designated uracil-DNA incision product (UIP), and a 5´-phosphate. The formation of UIP accords with an elimination (E2) reaction where deprotonation of C2´ occurs

Indexed as

Genes, ImmunoglobulinDNAHumansUracilUracil-DNA GlycosidaseDNAUracilUracil-DNA Glycosidaseclass switch recombinationcytosine deaminationhuman UNGimmunoglobulin diversificationsomatic hypermutation

Identifiers

PMID35003074
PMCPMC8730318
OpenAlexW4200432250

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.