ArticleFrontiers in neuroanatomy2021
High-Throughput Strategy for Profiling Sequential Section With Multiplex Staining of Mouse Brain.
Article in Frontiers in neuroanatomy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- spAttClu: a spatial domain clustering model leveraging spatially weighted graph attention and contrastive learning.Bioinformatics (Oxford, England) · 2026Article
- Article
- Parvalbumin Neurons in the Basal Forebrain Projecting to the Mammillary Nucleus Ameliorate Age-Related Cognitive Decline.International journal of molecular sciences · 2025Article
- Diversity of clustered protocadherin-α genes in neuronal identity and its role in short-term specific associative memory formation.Scientific reports · 2025Article
- SlicesMapi: An Interactive Three-Dimensional Registration Method for Serial Histological Brain Slices.Neuroinformatics · 2025Article
- Vulnerability of long-range inputome of basal forebrain in normal aging mice.Frontiers in aging neuroscience · 2025Article
- Three-dimensional mapping in multi-samples with large-scale imaging and multiplexed post staining.Communications biology · 2023Article
- The Connectome and Chemo-Connectome Databases for Mice Brain Connection Analysis.Frontiers in neuroanatomy · 2022Article
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Authors and funding
6 authors.
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Abstract
The brain modulates specific functions in its various regions. Understanding the organization of different cells in the whole brain is crucial for investigating brain functions. Previous studies have focused on several regions and have had difficulty analyzing serial tissue samples. In this study, we introduced a pipeline to acquire anatomical and histological information quickly and efficiently from serial sections. First, we developed a serial brain-slice-staining method to stain serial sections and obtained more than 98.5% of slices with high integrity. Subsequently, using the self-developed analysis software, we registered and quantified the signals of imaged sections to the Allen Mouse Brain Common Coordinate Framework, which is compatible with multimodal images and slant section planes. Finally, we validated the pipeline with immunostaining by analyzing the activity variance in the whole brain during acute stress in aging and young mice. By removing the problems resulting from repeated manual operations, this pipeline is widely applicable to serial brain slices from multiple samples in a rapid and convenient manner, which benefits to facilitate research in life sciences.
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