ArticleSaudi journal of biological sciences2022
Alcoholic fixation over formalin fixation: A new, safer option for morphologic and molecular analysis of tissues.
Article in Saudi journal of biological sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Ameliorative Role of fenugreek Oil Against Lead Acetate Trihydrate-Induced Hepatic, Renal and Testicular Injury in Wistar Albino Rats: Insights into Bcl-2 Expression and sodium/potassium balance.Biological trace element research · 2026Article
- Advancing three-dimensional tendon imaging using laboratory X-ray phase contrast techniques and refined sample preparation.Scientific reports · 2026Article
- Evaluation of lactic acid as a novel fixative for histological and neuroanatomical applications.Scientific reports · 2026Article
- Detailed microCT imaging protocol for ex vivo rat stomachs with comparative analysis.Scientific reports · 2025Article
- A New Wave of Ultrasound Phantoms Using Real Fixed Organs: Birth of the Danny Phantom.Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association · 2025Article
- Characterization of Cutaneous Radiation Syndrome in a Mouse Model Using [ 18 F]F- Fluorodeoxyglucose Positron Emission Tomography.Health physics · 2025Article
- A Method of Well-Spread Pachytene Chromosome Preparations for Plant Species with Large Genomes Suitable for the Immunolocalization of Meiotic Proteins.Methods and protocols · 2025Article
- Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo.Communications biology · 2025Article
- An in vitro model to measure the strength and stiffness of the extracellular matrix synthesized de novo by human fibroblasts.In vitro models · 2025Article
- Mastering the art of sectioning: a comprehensive guide to slide-microtome technology and histological applications.Frontiers in veterinary science · 2025Review
- Comparative analysis of fixation techniques for signal detection in avian embryos.Developmental biology · 2025Article
- Improved Preservation of Mouse Intestinal Tissue Using a Formalin/Acetic Acid Fixative and Quantitative Histological Analysis Using QuPath.Current protocols · 2024Article
- Optimization of Formaldehyde Fixative Concentration for Individual Blood Cells to Develop a Stabilized Blood Control for Automated Hematology Analyzers.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2024Article
- Effectiveness of fixation methods for wholemount immunohistochemistry across cellular compartments in chick embryos.bioRxiv : the preprint server for biology · 2024Article
- Effect of Fixatives and Fixation Period on Morphology and Immunohistochemistry of Feline Ovarian Tissue.Animals : an open access journal from MDPI · 2024Article
- An In-Vivo Study of Effects of Platelet-Rich Plasma on Transforming Growth Factor-β1 and Matrix Metalloprotein 9 Expression in Traumatic Ulcers with Diabetes Mellitus.European journal of dentistry · 2024Article
- The induction of bone formation by 3D-printed PLGA microsphere scaffolds in a calvarial orthotopic mouse model: a pilot study.Frontiers in bioengineering and biotechnology · 2024Article
- The clinical relevance of surgical specimens for RNA sequencing in lung cancer: a cohort study.Frontiers in oncology · 2024Article
- Article
- The hazardous effects of formalin and alcoholic fixative in mice: A public health perspective study.Saudi journal of biological sciences · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Formalin is a widely used fixative but there is potential public health risks to exposure. Besides, alcoholic fixation is advantageous over formalin fixation because of faster fixation, optimal preservation and safer workplace environment. Following fixation by EMA and 10% neutral buffered formalin (NBF), we analyzed the tissue morphology, antigenic stability, DNA and RNA quantity with quality (OD value). The findings of EMA fixing on both the tissue morphology and molecular characterization, were satisfactory. Specially, EMA was faster in penetration of tissues than NBF, fixed ideally as early as 8 h of fixation whereas improper fixation was evident for NBF. In Hematoxylin and Eosin (H & E) staining, better cellular details with stronger affinity for staining were observed. In immunohistochemistry, better antigenic stability was reported for EMA-fixed tissues. The nucleic acid analysis revealed that total genomic DNA and RNA yield from EMA fixed tissues were significantly higher (
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.