ArticleAntonie van Leeuwenhoek2022
Molecular analysis of genes involved in chitin degradation from the chitinolytic bacterium Bacillus velezensis.
Article in Antonie van Leeuwenhoek, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 28 citations in OpenAlex.
- Genomic and functional profiles of two Antarctic chitin-degrading Arthrobacter strains.Extremophiles : life under extreme conditions · 2026Article
- Integrative phylogenomic and pangenome landscape of Bacillus: insights from 10,000 genomes into taxonomy, functional potential, and biotechnological applications.World journal of microbiology & biotechnology · 2026Article
- Whole-genome characterization of halotolerant Enterobacter roggenkampii OSNO4 and its potential for climate-resilient agriculture.PloS one · 2026Article
- Recombinant expression and characterization of the family 5 cellulase fromBiochemistry and biophysics reports · 2025Article
- Novel domain-structure-containing chitinases A and B of Bacillus velezensis produced by recombinant Escherichia coli cells: Synergism on chitin degradation and their potential in suppressing Candida albicans cell germination.Journal, genetic engineering & biotechnology · 2024Article
- Exploring the Genomic Landscape ofCurrent issues in molecular biology · 2024Article
- Analysis of antimicrobial biological activity of a marine Bacillus velezensis NDB.Archives of microbiology · 2024Article
- Data on gene cloning, expression, purification, and characterization of the glycoside hydrolase family 11 fromData in brief · 2024Article
- Complete genome sequence data of chitin-degradingData in brief · 2023Article
- Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy againstPeerJ · 2023Article
- Bacterial chitinases: genetics, engineering and applications.World journal of microbiology & biotechnology · 2022Review
- Taxonomic and functional profiles ofData in brief · 2022Article
- Rhizosphere microbiome dataset of Robusta coffee (Coffea canephora L.) grown in the Central Highlands, Vietnam, based on 16S rRNA metagenomics analysis.Data in brief · 2022Article
- Analysis of endophytic microbiome dataset from roots of black pepper (Data in brief · 2022Article
- Expression, purification, and basic properties of a novel domain structure possessing chitinase from Escherichia coli carrying the family 18 chitinase gene of Bacillus velezensis strain RB.IBE29.Molecular biology reports · 2022Article
- Soil microbiome dataset from Yok Don national park in the Central Highlands region of Vietnam.Data in brief · 2022Article
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6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Bacillus velezensis RB.IBE29 is a potent biocontrol agent with high chitinase activity isolated from the rhizosphere of black pepper cultivated in the Central Highlands, Vietnam. Genome sequences revealed that this species possesses some GH18 chitinases and AA10 protein(s); however, these enzymes have not been experimentally characterized. In this work, three genes were identified from the genomic DNA of this bacterium and cloned in Escherichia coli. Sequence analysis exhibited that the ORF of chiA consists of 1,203 bp and encodes deduced 45.46 kDa-chitinase A of 400 aa. The domain structure of chitinase A is composed of a CBM 50 domain at the N-terminus and a catalytic domain at the C-terminus. The ORF of chiB includes 1,263 bp and encodes deduced 47.59 kDa-chitinase B of 420 aa. Chitinase B consists of two CBM50 domains at the N-terminus and a catalytic domain at the C-terminus. The ORF of lpmo10 is 621 bp and encodes a deduced 22.44 kDa-AA10 protein, BvLPMO10 of 206 aa. BvLPMO10 contains a signal peptide and an AA10 catalytic domain. Chitinases A and B were grouped into subfamily A of family 18 chitinases. Amino acid sequences in their catalytic domains lack aromatic residues (Trp, Phe, Tyr) probably involved in processivity and substrate binding compared with well-known bacterial GH18 chitinases. chiB was successfully expressed in E. coli. Purified rBvChiB degraded insoluble chitin and was responsible for inhibition of fungal spore-germination and egg hatching of plant-parasitic nematode. This is the first report describing the analysis of the chitinase system from B. velezensis.
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