ArticleBioprocess and biosystems engineering2022
High level production of stable human serum albumin in Pichia pastoris and characterization of the recombinant product.
Article in Bioprocess and biosystems engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 22 citations in OpenAlex.
- A New Strategy of Alternating Current Magnetic Field-Assisted Three-Phase Partitioning (ACMF-TPP) for the Concentration and Partial Purification of Recombinant Prepromelittin Produced by Komagataella phaffii.Biotechnology and applied biochemistry · 2026Article
- A Pichia pastoris-based platform for large-scale production of functional recombinant human serum albumin with therapeutic efficacy.Bioprocess and biosystems engineering · 2026Article
- A Simple Method to Produce Recombinant Mammalian Serum Albumins inInternational journal of molecular sciences · 2026Article
- Animal-derived components in cultivated meat research and their alternatives.NPJ science of food · 2025Review
- Fetal bovine serum: how to leave it behind in the pursuit of more reliable science.Frontiers in toxicology · 2025Review
- Optimization of dilution rate and mixed carbon feed for continuous production of recombinant plant sucrose:sucrose 1-fructosyltransferase in Komagataella phaffii.Bioprocess and biosystems engineering · 2024Article
- A self-inducible heterologous protein expression system in3 Biotech · 2024Article
- Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells.MethodsX · 2024Article
- Research Advances in Fusion Protein-Based Drugs for Diabetes Treatment.Diabetes, metabolic syndrome and obesity : targets and therapy · 2024Review
- Affinity-based and in a single step purification of recombinant horseradish peroxidase A2A isoenzyme produced by Pichia pastoris.Bioprocess and biosystems engineering · 2023Article
- Enhancing xylanase expression of Komagataella phaffii induced by formate through Mit1 co-expression.Bioprocess and biosystems engineering · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human serum albumin (HSA) is an important therapeutic used in clinical settings for restoration of blood volume and treatment of chemotherapy induced neutropenia. Currently sourced from human serum, it carries the risk of contamination with viruses. The production of stable extracellular recombinant (r)HSA was achieved at nearly 1 g/L at shake-flask level in Pichia pastoris (syn. Komagataella phaffii) containing a three-copy containing HSA expression cassette, prepared in vitro. The HSA specific transcripts were increased by 1.82- to 2.46-fold in the three-copy containing clones indicating increased transcript levels to result in enhanced production of extracellular rHSA. The purified rHSA displayed secondary structure, zeta potential, size distribution and biological efficacy that matched with that of the commercial HSA. Cultivation strategy was developed at bioreactor level for the single HSA expression cassette containing recombinant which led to productivity of 300 mg/L/d of rHSA with minimum proteolytic cleavage.
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