Evidence map›Paper›PMID 34999816›Full record

ArticleCardiovascular research2022

Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC Working Group on Myocardial Function and the ESC Working Group on Cellular Biology of the Heart.

Jolanda van der Velden, Folkert W Asselbergs, Jeroen Bakkers, Sandor Batkai, Luc Bertrand, Connie R Bezzina, Ilze Bot, Bianca J J M Brundel, Lucie Carrier, Steven Chamuleau and 51 more

Abstract readConsensus Statement
PubMed Full text at PMC Publisher
In one paragraph

Article in Cardiovascular research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  4. Optogenetic tachypacing facilitates burst-induced arrhythmia in stem cell-derived human atrial engineered heart tissue.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

61 authors.

Jolanda van der VeldenAmsterdam UMC, Vrije Universiteit, Physiology, Amsterdam Cardiovascular Science, Amsterdam, The Netherlands.ORCID 0000-0001-5224-5788
Folkert W AsselbergsDivision Heart & Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.ORCID 0000-0002-1692-8669
Jeroen BakkersHubrecht Institute-KNAW and University Medical Centre Utrecht, Utrecht, The Netherlands.ORCID 0000-0002-9418-0422
Sandor BatkaiHannover Medical School, Institute of Molecular and Translational Therapeutic Strategies, Hannover, Germany.
Luc BertrandHannover Medical School, Institute of Molecular and Translational Therapeutic Strategies, Hannover, Germany.ORCID 0000-0003-0655-7099
Connie R BezzinaUniversité catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Pole of Cardiovascular Research, Brussels, Belgium.ORCID 0000-0002-0633-3514
Ilze BotHeart Center, Department of Experimental Cardiology, Amsterdam UMC, Location Academic Medical Center, Amsterdam Cardiovascular Sciences, University of Amsterdam, Amsterdam, The Netherlands.ORCID 0000-0002-1242-1959
Bianca J J M BrundelAmsterdam UMC, Vrije Universiteit, Physiology, Amsterdam Cardiovascular Science, Amsterdam, The Netherlands.ORCID 0000-0002-4768-234X
Lucie CarrierInstitute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany.ORCID 0000-0001-8776-5820
Steven ChamuleauAmsterdam UMC, Heart Center, Cardiology, Amsterdam Cardiovascular Science, Amsterdam, The Netherlands.
Michele CiccarelliDepartment of Medicine, Surgery and Odontology, University of Salerno, Fisciano (SA), Italy.ORCID 0000-0003-2379-1960
Dana DawsonDepartment of Cardiology, Aberdeen Cardiovascular and Diabetes Centre, Aberdeen Royal Infirmary and University of Aberdeen, Aberdeen, UK.ORCID 0000-0003-2815-4469
Sean M DavidsonThe Hatter Cardiovascular Institute, University College London, 67 Chenies Mews, London WC1E 6HX, UK.ORCID 0000-0001-5182-4980
Andreas DendorferWalter-Brendel-Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-University, Munich, Germany.ORCID 0000-0002-7806-312X
Dirk J DunckerDivision of Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.ORCID 0000-0003-2836-2241
Thomas EschenhagenInstitute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg Eppendorf, Hamburg, Germany.
Larissa FabritzDZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck, Hamburg, Germany.ORCID 0000-0002-9241-1733
Ines Falcão-PiresUnIC - Cardiovascular Research and Development Centre, Department of Surgery and Physiology, Faculty of Medicine, University of Porto, Portugal.
Péter FerdinandyCardiometabolic Research Group and MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
Mauro GiaccaDepartment of Medicine, Surgery and Health Sciences and Cardiovascular Department, Centre for Translational Cardiology, Azienda Sanitaria Universitaria Integrata Trieste, Trieste, Italy.ORCID 0000-0003-2927-7225
Henrique GiraoUniv Coimbra, Center for Innovative Biomedicine and Biotechnology, Faculty of Medicine, Coimbra, Portugal.ORCID 0000-0002-5786-8447
Can Gollmann-TepeköylüDepartment of Cardiac Surgery, Medical University of Innsbruck, Innsbruck, Austria.ORCID 0000-0001-6584-3488
Mariann GyongyosiDivision of Cardiology, Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria.
Tomasz J GuzikInstutute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK.ORCID 0000-0003-2012-1187
Nazha HamdaniDivision Cardiology, Molecular and Experimental Cardiology, Ruhr University Bochum, Bochum, Germany.ORCID 0000-0002-3053-0008
Stephane HeymansDepartment of Cardiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University Medical Centre, Maastricht University, Maastricht, The Netherlands.
Andres HilfikerDepartment for Cardiothoracic, Transplant, and Vascular Surgery, Hannover Medical School, Hannover, Germany.ORCID 0000-0002-7123-2410
Denise Hilfiker-KleinerDepartment for Cardiology and Angiology, Hannover Medical School, Hannover, Germany.ORCID 0000-0001-6065-9649
Alfons G HoekstraComputational Science Lab, Informatics Institute, Faculty of Science, University of Amsterdam, Amsterdam, the Netherlands.ORCID 0000-0002-3955-2449
Jean-Sébastien HulotUniversité de Paris, INSERM, PARCC, F-75015 Paris, France.ORCID 0000-0001-5463-6117
Diederik W D KusterAmsterdam UMC, Vrije Universiteit, Physiology, Amsterdam Cardiovascular Science, Amsterdam, The Netherlands.ORCID 0000-0003-1498-6862
Linda W van LaakeDivision Heart & Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.
Sandrine LecourDepartment of Medicine, Hatter Institute for Cardiovascular Research in Africa and Cape Heart Institute, University of Cape Town, Cape Town, South Africa.ORCID 0000-0003-1942-8974
Tim LeinerDepartment of Radiology, Utrecht University Medical Center, Utrecht, the Netherlands.ORCID 0000-0003-1885-5499
Wolfgang A LinkeInstitute of Physiology II, University of Muenster, Robert-Koch-Str. 27B, 48149 Muenster, Germany.ORCID 0000-0003-0801-3773
Joost LumensDepartment of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands.ORCID 0000-0001-8129-7384
Esther LutgensExperimental Vascular Biology Division, Department of Medical Biochemistry, University of Amsterdam, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, Amsterdam, The Netherlands.ORCID 0000-0002-2609-5744
Rosalinda MadonnaDepartment of Pathology, Cardiology Division, University of Pisa, 56124 Pisa, Italy.ORCID 0000-0001-6455-2777
Lars MaegdefesselDZHK, Partner Site Munich Heart Alliance, Munich, Germany.ORCID 0000-0001-5228-2634
Manuel MayrKing's British Heart Foundation Centre, King's College London, London, UK.ORCID 0000-0002-0597-829X
Peter van der MeerDepartment of Cardiology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.ORCID 0000-0002-9705-4413
Robert PassierDepartment of Applied Stem Cell Technologies, TechMed Centre, University of Twente, 7500AE Enschede, The Netherlands.ORCID 0000-0003-4312-9296
Filippo PerbelliniHannover Medical School, Institute of Molecular and Translational Therapeutic Strategies, Hannover, Germany.ORCID 0000-0003-1540-2777
Cinzia PerrinoDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.ORCID 0000-0002-2274-0048
Maurizio PesceUnità di Ingegneria Tissutale Cardiovascolare, Centro cardiologico Monzino, IRCCS, Milan, Italy.ORCID 0000-0002-3097-8961
Silvia PrioriMolecular Cardiology, Istituti Clinici Scientifici Maugeri, Pavia, Italy.
Carol Ann RemmeUniversité catholique de Louvain, Institut de Recherche Expérimentale et Clinique, Pole of Cardiovascular Research, Brussels, Belgium.ORCID 0000-0003-0095-0084
Bodo RosenhahnInstitute for information Processing, Leibniz University of Hanover, 30167 Hannover, Germany.
Ulrich SchottenDepartment of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.ORCID 0000-0003-1532-3315
Rainer SchulzInstitute of Physiology, Justus Liebig University Giessen, Giessen, Germany.ORCID 0000-0003-3017-0476
Karin R SipidoDepartment of Cardiovascular Sciences, KU Leuven, 3000 Leuven, Belgium.
Joost P G SluijterExperimental Cardiology Laboratory, Department of Cardiology, Regenerative Medicine Center Utrecht, Circulatory Health Laboratory, Utrecht University, University Medical Center Utrecht, Utrecht, The Netherlands.ORCID 0000-0003-2088-9102
Frank van SteenbeekDivision Heart & Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.
Sabine SteffensInstitute for Cardiovascular Prevention, Ludwig-Maximilians-Universität München (LMU), Munich, Germany.ORCID 0000-0002-6892-9751
Cesare M TerraccianoNational Heart & Lung Institute, Imperial College London, London, UK.
Carlo Gabriele TocchettiCardio-Oncology Unit, Department of Translational Medical Sciences, Center for Basic and Clinical Immunology Research (CISI), Interdepartmental Center for Clinical and Translational Research (CIRCET), Interdepartmental Hypertension Research Center (CIRIAPA), Federico II University, Naples, Italy.ORCID 0000-0001-5983-688X
Patricia VlasmanAmsterdam UMC, Vrije Universiteit, Physiology, Amsterdam Cardiovascular Science, Amsterdam, The Netherlands.ORCID 0000-0003-3713-4216
Kak Khee YeungAmsterdam UMC, Vrije Universiteit, Surgery, Amsterdam Cardiovascular Science, Amsterdam, The Netherlands.ORCID 0000-0002-8455-286X
Serena ZacchignaDepartment of Medicine, Surgery and Health Sciences and Cardiovascular Department, Centre for Translational Cardiology, Azienda Sanitaria Universitaria Integrata Trieste, Trieste, Italy.ORCID 0000-0001-6705-3076
Dayenne ZwaagmanAmsterdam UMC, Heart Center, Cardiology, Amsterdam Cardiovascular Science, Amsterdam, The Netherlands.
Thomas ThumHannover Medical School, Institute of Molecular and Translational Therapeutic Strategies, Hannover, Germany.ORCID 0000-0003-4360-1511

Funding

Computational and functional strategies to decipher lncRNAs in human atherosclerosisR01HL150359 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI LI, MINGYAO, REILLY, MUREDACH P · 2020 to 2023
$2.7M
British Heart Foundation CH/16/3/32406British Heart Foundation FS/18/37/33642British Heart Foundation FS/RTF/20/30009British Heart Foundation FS/RTF/20/30009, NH/19/1/34595, PG/18/35/33786, CS/17/4/32960, PG/15/88/31780, and PG/17/64/33205British Heart Foundation PG/15/88/31780British Heart Foundation PG/17/64/33205British Heart Foundation PG/18/44/33790British Heart Foundation RG/16/14/32397National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/T001488/1NHLBI NIH HHS R01 HL150359
6 · The paper itself

Abstract

Cardiovascular diseases represent a major cause of morbidity and mortality, necessitating research to improve diagnostics, and to discover and test novel preventive and curative therapies, all of which warrant experimental models that recapitulate human disease. The translation of basic science results to clinical practice is a challenging task, in particular for complex conditions such as cardiovascular diseases, which often result from multiple risk factors and comorbidities. This difficulty might lead some individuals to question the value of animal research, citing the translational 'valley of death', which largely reflects the fact that studies in rodents are difficult to translate to humans. This is also influenced by the fact that new, human-derived in vitro models can recapitulate aspects of disease processes. However, it would be a mistake to think that animal models do not represent a vital step in the translational pathway as they do provide important pathophysiological insights into disease mechanisms particularly on an organ and systemic level. While stem cell-derived human models have the potential to become key in testing toxicity and effectiveness of new drugs, we need to be realistic, and carefully validate all new human-like disease models. In this position paper, we highlight recent advances in trying to reduce the number of animals for cardiovascular research ranging from stem cell-derived models to in situ modelling of heart properties, bioinformatic models based on large datasets, and state-of-the-art animal models, which show clinically relevant characteristics observed in patients with a cardiovascular disease. We aim to provide a guide to help researchers in their experimental design to translate bench findings to clinical routine taking the replacement, reduction, and refinement (3R) as a guiding concept.

Indexed as

Cardiovascular DiseasesAnimalsHumansModels, AnimalResearch DesignBig dataBioinformaticsCardiovascular diseaseComorbiditiesiPSCMultiomicsNetwork medicineTissue engineering

Identifiers

PMID34999816
PMCPMC9732557

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.