Evidence map›Paper›PMID 34997871›Full record

ArticleMolecular biology reports2022

Melatonin inhibits autophagy in TM3 cells via AKT/FOXO1 pathway.

Zhiqiang Li, Hongtao Wang, Kaiyan Zhang, Jing Zhao, Hongyu Liu, Xin Ma, Jing Guo, Jun Wang, Wenfa Lu

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Role of Melatonin in Bovine Reproductive Biotechnology.Molecules (Basel, Switzerland) · 2023
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Zhiqiang LiJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China.
Hongtao WangJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China.
Kaiyan ZhangJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China.
Jing ZhaoJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China.
Hongyu LiuJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China.
Xin MaJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China.
Jing GuoJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China.
Jun WangJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China. junwang2004@126.com.
Wenfa LuJoint Laboratory of the Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, 130118, Changchun, Jilin, China. wenfa2004@163.com.ORCID http://orcid.org/0000-0002-8303-2117
Jilin Agricultural University · CNJilin University · CN

Funding

Jilin Province Philosophy and Social Science Planning Fund Office 20200301031RQNational Basic Research Program of China (973 Program) 2018YFE0122000National Natural Science Foundation of China 31772600
6 · The paper itself

Abstract

backgroundMelatonin can regulate apoptosis and autophagy of mouse Leydig cells, but its specific mechanism is still unclear.

methodsIn this study, we used the TM3 cell line as the research object, and used H2O2 to induce autophagy. After adding 10 ng/ml melatonin, we used qRT-PCR and western-blot to detect autophagy-related gene and protein expression, and flow cytometry to detect cellular ROS level.

resultsThe results showed that melatonin can significantly inhibit the occurrence of autophagy, accompanied by a significant decrease in the expression of Becn1, LC3, and FOXO1 (P < 0.05), a significant increase in the expression of p62 and pAKT (P < 0.05), and a significant decrease in ROS level (P < 0.05). After added the inhibitor of AKT perifosine, the effect of melatonin on inhibiting autophagy was reversed. On this basis, we used small RNA interference technology to knock down the expression of FOXO1, and found that there was no significant change of the expression of genes and proteins related to autophagy and ROS level.

conclusionsIn summary, melatonin can inhibit H2O2-induced autophagy in TM3 cells through the AKT/FOXO1 pathway.

Indexed as

MelatoninAnimalsApoptosisAutophagyForkhead Box Protein O1Hydrogen PeroxideMaleMiceProto-Oncogene Proteins c-aktForkhead Box Protein O1Foxo1 protein, mouseHydrogen PeroxideMelatoninProto-Oncogene Proteins c-aktAKTAutophagyFOXO1MelatoninMouseTM3 cells

Identifiers

PMID34997871
OpenAlexW4206140193

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.