Evidence map›Paper›PMID 34997082›Full record

ArticleScientific reports2022

MiR-29b may suppresses peritoneal metastases through inhibition of the mesothelial-mesenchymal transition (MMT) of human peritoneal mesothelial cells.

Yuki Kimura, Hideyuki Ohzawa, Hideyo Miyato, Yuki Kaneko, Akira Saito, Kazuya Takahashi, Mineyuki Tojo, Hironori Yamaguchi, Kentaro Kurashina, Shin Saito and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. MicroRNA in cancer therapy: breakthroughs and challenges in early clinical applications.Journal of experimental & clinical cancer research : CR · 2025
    Review
  6. Article
  7. Article
  8. Myocardin regulates fibronectin expression and secretion from human pleural mesothelial cells.American journal of physiology. Lung cellular and molecular physiology · 2024
    Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 1 institution in 1 country.

Yuki KimuraDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Hideyuki OhzawaDepartment of Clinical Oncology, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Hideyo MiyatoDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Yuki KanekoDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Akira SaitoDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Kazuya TakahashiDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Mineyuki TojoDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Hironori YamaguchiDepartment of Clinical Oncology, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Kentaro KurashinaDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Shin SaitoDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Yoshinori HosoyaDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Alan Kawarai LeforDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Naohiro SataDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan.
Joji KitayamaDepartment of Surgery, Jichi Medical University, Shimotsuke, Tochigi, Japan. kitayama@jichi.ac.jp.
Jichi Medical University · JP

Funding

Japan Society for the Promotion of Science 20K07704
6 · The paper itself

Abstract

Peritoneal dissemination is a major metastatic pathway for gastrointestinal and ovarian malignancies. The miR-29b family is downregulated in peritoneal fluids in patients with peritoneal metastases (PM). We examined the effect of miR-29b on mesothelial cells (MC) which play critical a role in the development of PM through mesothelial-mesenchymal transition (MMT). Human peritoneal mesothelial cells (HPMCs) were isolated from surgically resected omental tissue and MMT induced by stimulation with 10 ng/ml TGF-β1. MiR-29b mimics and negative control miR were transfected by lipofection using RNAiMAX and the effects on the MMT evaluated in vitro. HPMC produced substantial amounts of miR-29b which was markedly inhibited by TGF-β1. TGF-β1 stimulation of HPMC induced morphological changes with decreased expression of E-cadherin and calretinin, and increased expression of vimentin and fibronectin. TGF-β1 also enhanced proliferation and migration of HPMC as well as adhesion of tumor cells in a fibronectin dependent manner. However, all events were strongly abrogated by simultaneous transfection of miR-29b. MiR-29b inhibits TGF-β1 induced MMT and replacement of miR-29b in the peritoneal cavity might be effective to prevent development of PM partly through the effects on MC.

Indexed as

Epithelial-Mesenchymal TransitionAnimalsAntigens, CDCadherinsCalbindin 2Cell AdhesionCell Line, TumorCell MovementCell ProliferationCoculture TechniquesEpithelial CellsFibronectinsHumansMiceMice, Inbred C57BLMicroRNAsAntigens, CDCadherinsCALB2 protein, humanCalbindin 2CDH1 protein, humanFibronectinsMicroRNAsMIRN29B1 microRNA, humanTransforming Growth Factor beta1VimentinVIM protein, human

Identifiers

PMID34997082
PMCPMC8742040
OpenAlexW4206716465

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.