ArticleScientific reports2022
The NarX-NarL two-component system regulates biofilm formation, natural product biosynthesis, and host-associated survival in Burkholderia pseudomallei.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Airborne Bacterial and Eukaryotic Communities in Temuco, Chile, a City Dominated by Residential Wood Combustion.Life (Basel, Switzerland) · 2026Article
- The IrlS2-IrlR2 two-component system is a global regulator of biofilm formation, stress adaptation, and virulence inmSphere · 2026Article
- Recombinant Cytosolic Truncations of Histidine Kinases Retain Function for Targeted In Vitro Investigations.Microorganisms · 2026Article
- The PhoPQ two-component regulatory system as an important regulator of bacterial stress response.Molecular biology reports · 2025Review
- The histidine kinase NahK regulates denitrification and nitric oxide accumulation through RsmA inJournal of bacteriology · 2025Article
- Deletion of both anaerobic regulator genes fnr and narL compromises the colonization of Salmonella Typhimurium in mice model.World journal of microbiology & biotechnology · 2024Article
- Resistance to freezing conditions of endemic Antarctic polychaetes is enhanced by cryoprotective proteins produced by their microbiome.Science advances · 2024Article
- Increased triacylglycerol production in Rhodococcus opacus by overexpressing transcriptional regulators.Biotechnology for biofuels and bioproducts · 2024Article
- Acetylation of K188 and K192 inhibits the DNA-binding ability of NarL to regulateApplied and environmental microbiology · 2023Article
- NosP Detection of Heme ModulatesBiochemistry · 2023Article
- Article
- Biofilm Signaling, Composition and Regulation inJournal of microbiology and biotechnology · 2023Review
- Two-component systems regulate bacterial virulence in response to the host gastrointestinal environment and metabolic cues.Virulence · 2022Review
- Article
- Pseudomonas aeruginosa Initiates a Rapid and Specific Transcriptional Response during Surface Attachment.Journal of bacteriology · 2022Article
- Disruption of c-di-GMP Signaling Networks Unlocks Cryptic Expression of Secondary Metabolites during Biofilm Growth inApplied and environmental microbiology · 2022Article
- Nitrate- and Nitrite-Sensing Histidine Kinases: Function, Structure, and Natural Diversity.International journal of molecular sciences · 2021Review
- RegAB Homolog of Burkholderia pseudomallei is the Master Regulator of Redox Control and involved in Virulence.PLoS pathogens · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Burkholderia pseudomallei is a saprophytic bacterium endemic throughout the tropics causing severe disease in humans and animals. Environmental signals such as the accumulation of inorganic ions mediates the biofilm forming capabilities and survival of B. pseudomallei. We have previously shown that B. pseudomallei responds to nitrate and nitrite by inhibiting biofilm formation and altering cyclic di-GMP signaling. To better understand the roles of nitrate-sensing in the biofilm inhibitory phenotype of B. pseudomallei, we created in-frame deletions of narX (Bp1026b_I1014) and narL (Bp1026b_I1013), which are adjacent components of a conserved nitrate-sensing two-component system. We observed transcriptional downregulation in key components of the biofilm matrix in response to nitrate and nitrite. Some of the most differentially expressed genes were nonribosomal peptide synthases (NRPS) and/or polyketide synthases (PKS) encoding the proteins for the biosynthesis of bactobolin, malleilactone, and syrbactin, and an uncharacterized cryptic NRPS biosynthetic cluster. RNA expression patterns were reversed in ∆narX and ∆narL mutants, suggesting that nitrate sensing is an important checkpoint for regulating the diverse metabolic changes occurring in the biofilm inhibitory phenotype. Moreover, in a macrophage model of infection, ∆narX and ∆narL mutants were attenuated in intracellular replication, suggesting that nitrate sensing contributes to survival in the host.
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