ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2022
Short-term Housing in Metabolic Caging on Measures of Energy and Fluid Balance in Male C57BL/6J Mice (
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- β-Arrestin-2 Regulates Sodium Taste Processing.Hypertension (Dallas, Tex. : 1979) · 2026Article
- Diurnal regulation of urinary behavior and gene expression in aged mice.The journals of gerontology. Series A, Biological sciences and medical sciences · 2025Article
- Thermoneutral environment improves mouse welfare and reduces stress in metabolic cages.Lab animal · 2025Article
- Reutericyclin mitigates risperidone-induced suppression of anaerobic energy expenditure.American journal of physiology. Regulatory, integrative and comparative physiology · 2025Article
- Early-life sodium restriction programs autonomic dysfunction and salt sensitivity in male C57BL/6J mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2025Article
- Male Wistar Rats Chronically Fed with a High-Fat Diet Develop Inflammatory and Ionic Transport Angiotensin-(3-4)-Sensitive Myocardial Lesions but Preserve Echocardiographic Parameters.International journal of molecular sciences · 2024Article
- Long-term Metabolic Dysfunction Programming in Female Mice by Serial Moderate Restriction of a High-fat High-sucrose Diet.Endocrinology · 2024Article
- Krüppel-like factor 4 in transcriptional control of the three unique isoforms of Agouti-related peptide in mice.Physiological genomics · 2024Article
- Modulatory effects of estrous cycle on ingestive behaviors and energy balance in young adult C57BL/6J mice maintained on a phytoestrogen-free diet.American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Early-life sodium deprivation programs long-term changes in ingestive behaviors and energy expenditure in C57BL/6J mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2023Article
- Cardiometabolic Effects of DOCA-Salt in Mice Depend on Ambient Temperature.Hypertension (Dallas, Tex. : 1979) · 2023Article
- Prolonged fasting times reap greater geroprotective effects when combined with caloric restriction in adult female mice.Cell metabolism · 2023Article
- Cardiometabolic Consequences of Deleting the Regulator of G protein Signaling-2 (Hypertension (Dallas, Tex. : 1979) · 2022Article
- Cardiometabolic effects of DOCA-salt in male C57BL/6J mice are variably dependent on sodium and nonsodium components of diet.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- Methods for the ComprehensiveFrontiers in physiology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Metabolic caging is an important tool for quantitative urine and feces collection in rodents, although significant limitations and problems accompany its use. Despite strong opinions among investigators regarding the effects of metabolic caging on energy and fluid homeostasis, careful quantitative analysis of the impact of this caging type-particularly when used for mice-is lacking. The current study assessed the effects of metabolic caging, with or without modifications such as plastic platform inserts, on ingestive behaviors, energy expenditure, accuracy of urine and fecal collection, and ambulatory activities in male C57BL/6J mice. Housing mice in metabolic cages, regardless of platform inclusion, increased energy expenditure without modifying food intake, presumably due to the inability of mice to perform normal thermoregulatory behaviors (burrowing and huddling). Surprisingly, mice in metabolic cages actively avoided platforms, and the inclusion of platforms modified the behavior of the mice and had position-dependent effects that reduced the accuracy of urine collection. Moving mice from cohousing to individual housing in home cages also increased ingestive behaviors and energy expenditure. We conclude that single housing of male C57BL/6J mice increases energy expenditure, that this increase is potentiated in metabolic caging conditions, and that platforms in metabolic cages alter mouse behavior and urine collection. Additional future work is needed to determine the potential benefits of using higher ambient temperature for studies of mice in metabolic caging and whether the above effects occur in females and other strains of mice and other rodent species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.