Evidence map›Paper›PMID 34995523›Full record

ArticleAmerican journal of ophthalmology2022

Effects of Mitomycin-C and 5-Fluorouracil on Ocular Adnexal Sebaceous Carcinoma Cells.

Ryan A Gallo, Steven H Lang, Angela Gomez, Alfonso L Sabater, David T Tse, Daniel Pelaez, Andrew J Rong

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of ophthalmology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ryan A GalloFrom the Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center (R.A.G., S.H.L., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Ophthalmology (A.G., A.L.S., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Oculoplastic Surgery (D.T.T., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL.
Steven H LangFrom the Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center (R.A.G., S.H.L., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Ophthalmology (A.G., A.L.S., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Oculoplastic Surgery (D.T.T., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL.
Angela GomezFrom the Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center (R.A.G., S.H.L., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Ophthalmology (A.G., A.L.S., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Oculoplastic Surgery (D.T.T., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL.
Alfonso L SabaterFrom the Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center (R.A.G., S.H.L., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Ophthalmology (A.G., A.L.S., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Oculoplastic Surgery (D.T.T., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL.
David T TseFrom the Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center (R.A.G., S.H.L., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Ophthalmology (A.G., A.L.S., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Oculoplastic Surgery (D.T.T., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL.
Daniel PelaezFrom the Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center (R.A.G., S.H.L., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Ophthalmology (A.G., A.L.S., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Oculoplastic Surgery (D.T.T., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL.
Andrew J RongFrom the Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center (R.A.G., S.H.L., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Ophthalmology (A.G., A.L.S., D.T.T., D.P., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL; Department of Oculoplastic Surgery (D.T.T., A.J.R.), Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL. Electronic address: ajr211@med.miami.edu.
University of Miami · US

Funding

Shared Equipment ModuleP30EY014801 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI Victor L Perez · 2004 to 2026
$12.2M
Medical Scientist Training ProgramT32GM112601 · NIGMS · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI LEMMON, SANDRA K. · 2017 to 2021
$1.9M
NEI NIH HHS P30 EY014801NIGMS NIH HHS T32 GM112601
6 · The paper itself

Abstract

purposeTo investigate the effects of mitomycin-C (MMC) and 5-fluorouracil (5-FU) on the viability, proliferation, and migratory capacity of cultured ocular adnexal sebaceous carcinoma (SC) cells.

designLaboratory investigation.

methodsHuman SC cell lines (Bascom Palmer 50 and 52 [BP50 and BP52]) and human limbal stem cells (LSCs) were treated with various concentrations of MMC and 5-FU. Cytotoxicity was assessed with the tetrazolium MTT colorimetric viability assay on normal corneal vs tumor cells. Growth curves and scratch assays were performed to characterize the effects of these chemotherapeutic agents on SC proliferation and migration, respectively.

resultsMMC decreased BP52 cell viability in a dose-dependent manner with a half-maximal effective dose (EC

conclusionsOur in vitro data corroborate clinical observations that MMC is efficacious for treating ocular adnexal SC, albeit at the expense of LSC viability. Our findings also demonstrate that topical 5-FU exhibits antiproliferative effects that supersede its cancer-killing and antimigratory effects on cultured SC cells.

Indexed as

CarcinomaEye NeoplasmsCells, CulturedCell SurvivalFluorouracilHumansMitomycinFluorouracilMitomycin

Identifiers

PMID34995523
PMCPMC9250945
OpenAlexW4205930738

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.