ArticleNature biotechnology2022
Engineering the amoeba Dictyostelium discoideum for biosynthesis of a cannabinoid precursor and other polyketides.
Article in Nature biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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14 citing papers in PubMed, 30 citations in OpenAlex.
- Engineering metazoan fatty acid synthase to control chain length applied in yeast.Nature chemical biology · 2026Article
- Recent innovations in cannabinoid chemistry, biology, and biosynthesis.Current opinion in biotechnology · 2025Review
- The Amoeba Dictyostelium discoideum as Novel Production Host for Complex Substances.Advances in biochemical engineering/biotechnology · 2025Review
- Identification and Optimization of more Efficient Olivetolic Acid Synthases.Cannabis and cannabinoid research · 2024Article
- Optimized psilocybin production in tryptophan catabolism-repressed fungi.Microbial biotechnology · 2024Article
- Article
- Evaluation of Alternative Colony Hybridization Methods for Pathogenic Vibrios.Foods (Basel, Switzerland) · 2023Article
- Reshaping the 2-Pyrone Synthase Active Site for Chemoselective Biosynthesis of Polyketides.Angewandte Chemie (International ed. in English) · 2023Article
- Article
- Metabolic Engineering ofMetabolites · 2022Article
- Yellow polyketide pigment suppresses premature hatching in social amoeba.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Scale-up of an amoeba-based process for the production of the cannabinoid precursor olivetolic acid.Microbial cell factories · 2022Article
- Generating polyketide diversity inProceedings. Biological sciences · 2022Article
- CRISPR/Cas9-based genome-wide screening of Dictyostelium.Scientific reports · 2022Article
Corrections and comments
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- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aromatic polyketides are natural polyphenolic compounds with a broad spectrum of pharmacological activities. Production of those metabolites in the model organisms Escherichia coli and Saccharomyces cerevisiae has been limited by the extensive cellular engineering needed for the coordinated biosynthesis of polyketides and their precursors. In contrast, the amoeba Dictyostelium discoideum is a native producer of secondary metabolites and harbors a wide, but largely unexplored, repertoire of genes for the biosynthesis of polyketides and terpenoids. Here we present D. discoideum as an advantageous chassis for the production of aromatic polyketides. By expressing its native and cognate plant polyketide synthase genes in D. discoideum, we demonstrate production of phlorocaprophenone, methyl-olivetol, resveratrol and olivetolic acid (OA), which is the central intermediate in the biosynthesis of cannabinoids. To facilitate OA synthesis, we further engineered an amoeba/plant inter-kingdom hybrid enzyme that produced OA from primary metabolites in two enzymatic steps, providing a shortcut in a synthetic cannabinoid pathway using the D. discoideum host system.
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