Evidence map›Paper›PMID 34989928›Full record

ArticleBiodegradation2022

LcaR: a regulatory switch from Pseudomonas aeruginosa for bioengineering alkane degrading bacteria.

Erandika H Hemamali, Laksiri P Weerasinghe, Hideaki Tanaka, Genji Kurisu, Inoka C Perera

Abstract read
PubMed Publisher
In one paragraph

Article in Biodegradation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Antibiotics (Basel, Switzerland) · 2024
    Review
  3. Structure and Function of Alkane Monooxygenase (AlkB).Accounts of chemical research · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Erandika H HemamaliSynthetic Biology Laboratory, Department of Zoology and Environment Sciences, Faculty of Science, University of Colombo, Colombo, Sri Lanka.ORCID 0000-0003-0249-2603
Laksiri P WeerasingheDepartment of Chemistry, Faculty of Applied Science, University of Sri Jayewardenapura, Colombo, Sri Lanka.ORCID 0000-0003-1255-1101
Hideaki TanakaInstitute for Protein Research, Osaka University, Osaka, Japan.ORCID 0000-0003-2035-954X
Genji KurisuInstitute for Protein Research, Osaka University, Osaka, Japan.ORCID 0000-0002-5354-0807
Inoka C PereraSynthetic Biology Laboratory, Department of Zoology and Environment Sciences, Faculty of Science, University of Colombo, Colombo, Sri Lanka. icperera@sci.cmb.ac.lk.ORCID 0000-0002-2496-7385

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Application of genetically engineered bacterial strains for biodegradation of hydrocarbons is a sustainable solution for treating pollutants as well as in industrial applications. However, the process of bioengineering should be carefully carried out to optimize the output. Investigation of regulatory genes for bioengineering is essential for developing synthetic circuits for effective biocatalysts. Here we focus on LcaR, a putative transcriptional regulator affecting the expression of alkB2 and lcaR operon that has a high potential to become a tool in designing such pathways. Four LcaR dimers bind specifically to the upstream regulatory region where divergent promoters of alkB2 and lcaR genes are located with high affinity at a K

Indexed as

AlkanesPseudomonas aeruginosaBacterial ProteinsBiodegradation, EnvironmentalBioengineeringAlkanesBacterial ProteinsAlkane 1-monooxygenaseAlkane degradationDNA binding proteinPseudomonas aeruginosaTranscription factor

Identifiers

PMID34989928

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.