ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022
Light-Triggered In Situ Biosynthesis of Artificial Melanin for Skin Protection.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- All-small-molecule Hydrogels for Safe and Efficient Photoprotection.Nature communications · 2026Article
- Natural protection against oxidative stress in human skin melanocytes.Communications biology · 2025Review
- Injectable Endoplasmin-Loaded Lipid Nanoparticles-Hydrogel Composite for Cartilage Regeneration.Tissue engineering and regenerative medicine · 2025Article
- Genetic Code Expansion: Recent Developments and Emerging Applications.Chemical reviews · 2025Review
- Recent Advances in Nanotechnology-Mediated Noninvasive Transdermal and Topical Delivery of Proteins.Small science · 2024Article
- Development of a Stable Peptide-Major Histocompatibility Complex (MHC) via Sortase and Click Chemistry.ACS pharmacology & translational science · 2024Article
- Paintable Decellularized-ECM Hydrogel for Preventing Cardiac Tissue Damage.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Tyrosinases: a family of copper-containing metalloenzymes.Chemtexts · 2024Article
- Melanin/melanin-like nanoparticles: As a naturally active platform for imaging-guided disease therapy.Materials today. Bio · 2023Review
- Bioinspired polydopamine nanoparticles as efficient antioxidative and anti-inflammatory enhancers against UV-induced skin damage.Journal of nanobiotechnology · 2023Article
- Applications of genetic code expansion and photosensitive UAAs in studying membrane proteins.Open life sciences · 2023Review
- Synthesis of soluble melanin nanoparticles under acidic conditions usingRSC advances · 2022Article
- Light-Triggered In Situ Biosynthesis of Artificial Melanin for Skin Protection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
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Authors and funding
10 authors at 3 institutions in 5 countries.
Funding
Abstract
Tyrosinase-mediated melanin synthesis is an essential biological process that can protect skin from UV radiation and radical species. This work reports on in situ biosynthesis of artificial melanin in native skin using photoactivatable tyrosinase (PaTy). The I41Y mutant of Streptomyces avermitilis tyrosinase (SaTy) shows enzymatic activity comparable to that of wild-type SaTy. This Y41 is replaced with photocleavable o-nitrobenzyl tyrosine (ONBY) using the introduction of amber codon and ONBY-tRNA synthetase/tRNA pairs. The ONBY efficiently blocks the active site and tyrosinase activity is rapidly recovered by the photo-cleavage of ONBY. The activated PaTy successfully oxidizes L-tyrosine and tyramine-conjugated hyaluronic acid (HA_T) to synthesize melanin particles and hydrogel, respectively. To produce artificial melanin in living tissues, PaTy is encapsulated into lipid nanoparticles as an artificial melanosome. Using liposomes containing PaTy (PaTy_Lip), PaTy is transdermally delivered into ex vivo porcine skin and in vivo mouse skin tissues, thus achieving the in situ biosynthesis of artificial melanin for skin tissue protection under UV irradiation. The results of this study demonstrate that this biomimetic system can recapitulate the biosynthetic analogs of naturally occurring melanin. It should therefore be considered to be a promising strategy for producing protective biological molecules within living systems for tissue protection.
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