Evidence map›Paper›PMID 34988675›Full record

ArticleFunctional & integrative genomics2022

Genome-wide profiling of drought-tolerant Arabidopsis plants over-expressing chickpea MT1 gene reveals transcription factors implicated in stress modulation.

Sanoj Kumar, Ankita Yadav, Nasreen Bano, Arvind Kumar Dubey, Rita Verma, Ankesh Pandey, Anil Kumar, Sumit Bag, Sudhakar Srivastava, Indraneel Sanyal

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Article in Functional & integrative genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 22% of its field
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed, 4 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Sanoj KumarPlant Transgenic Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Uttar Pradesh, Lucknow, 226001, India.
Ankita YadavPlant Transgenic Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Uttar Pradesh, Lucknow, 226001, India.
Nasreen BanoPlant Transgenic Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Uttar Pradesh, Lucknow, 226001, India.
Arvind Kumar DubeyPlant Stress Laboratory, French Associates Institute for Agriculture and Biotechnology of Drylands, Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus 84990, Be'er Sheva, Israel.
Rita VermaPlant Transgenic Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Uttar Pradesh, Lucknow, 226001, India.
Ankesh PandeyPlant Transgenic Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Uttar Pradesh, Lucknow, 226001, India.
Anil KumarPlant Transgenic Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Uttar Pradesh, Lucknow, 226001, India.
Sumit BagPlant Transgenic Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Uttar Pradesh, Lucknow, 226001, India.
Sudhakar SrivastavaInstitute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, 221005, India.
Indraneel SanyalPlant Transgenic Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Uttar Pradesh, Lucknow, 226001, India. i.sanyal@nbri.res.in.ORCID http://orcid.org/0000-0002-2592-773X
National Botanical Research Institute · INBanaras Hindu University · INBen-Gurion University of the Negev · ILKumaun University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Drought, a major abiotic limiting factor, could be modulated with in-built reprogramming of plants at molecular level by regulating the activity of plant developmental processes, stress endurance and adaptation. The transgenic Arabidopsis thaliana over-expressing metallothionein 1 (MT1) gene of desi chickpea (Cicer arietinum L.) was subjected to transcriptome analysis. We evaluated drought tolerance of 7 days old plants of Arabidopsis thaliana in both wild-type (WT) as well as transgenic plants and performed transcriptome analysis. Our analysis revealed 24,737 transcripts representing 24,594 genes out of which 5,816 were differentially expressed genes (DEGs) under drought conditions and 841 genes were common in both genotypes. A total of 1251 DEGs in WT and 2099 in MT1 were identified in comparison with control. Out of the significant DEGs, 432 and 944 were upregulated, whereas 819 and 1155 were downregulated in WT and MT1 plants, respectively. The physiological and molecular parameters involving germination assay, root length measurements under different stress treatments and quantitative expression analysis of transgenic plants in comparison to wild-type were found to be enhanced. CarMT1 plants also demonstrated modulation of various other stress-responsive genes that reprogrammed themselves for stress adaptation. Amongst various drought-responsive genes, 24 DEGs showed similar quantitative expression as obtained through RNA sequencing data. Hence, these modulatory genes could be used as a genetic tool for understanding and delineating the mechanisms for fine-tuning of stress responses in crop plants.

Indexed as

ArabidopsisCicerDroughtsGene Expression ProfilingGene Expression Regulation, PlantMetallothioneinPlants, Genetically ModifiedStress, PhysiologicalTranscription FactorsMetallothioneinTranscription FactorsAbiotic stressArabidopsis thalianaDroughtHeterologous expressionMetallothioneinTranscriptome

Identifiers

PMID34988675
OpenAlexW4205344673

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.