ArticleAnnals of translational medicine2021
Characterization of microRNAs in spent culture medium associated with human embryo quality and development.
Article in Annals of translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Non-Coding RNAs as Biomarkers for Embryo Quality and Pregnancy Outcomes: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2023Pooled it
- "Old and new" frontiers in embryo viability assessment: current practices and introduction of emerging approaches.Biology of reproduction · 2026Review
- Small RNA sequencing in individually selected sperm: Biomarkers for male subfertility and predictors of pregnancy success.Non-coding RNA research · 2026Article
- Transcriptomics empower assisted reproductive technology in decoding gamete and embryo development potential: a review.Journal of assisted reproduction and genetics · 2025Review
- MiR-432-5p was involved in gestational diabetes mellitus by targeting COL5A2 regulation.Irish journal of medical science · 2025Article
- Article
- New approaches in the analysis of spent embryo culture media in the IVF process.Archives of gynecology and obstetrics · 2025Article
- Noninvasive Biomarkers of Human Embryo Developmental Potential.International journal of molecular sciences · 2025Review
- Up-regulated microRNAs in blastocoel fluid of human implanted embryos could control circuits of pluripotency and be related to embryo competence.Journal of assisted reproduction and genetics · 2025Article
- Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods.Clinical and experimental reproductive medicine · 2025Article
- Embryo-secreted microRNAs enable non-invasive assessment of chromosomal status and viability inFrontiers in cell and developmental biology · 2025Review
- miRNAs in Follicular and Oviductal Fluids Support Global DNA Demethylation in Early-Stage Embryos.International journal of molecular sciences · 2024Article
- Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology.Communications medicine · 2024Article
- A validated model for individualized prediction of pregnancy outcome in woman after fresh cycle of Day 5 single blastocyst transfer.Scientific reports · 2023Article
- Decoding the Follicular Fluid Microenvironment: Integrative Biomarkers and Multiomics Signatures to Predict IVF Outcomes.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGiven implantation failure limits the improvement of in vitro fertilization (IVF) success rates, there is an urgency to identify potential biomarkers for embryo quality and predict the outcomes of IVF-embryo transfer (IVF-ET).
methodsUsing RNA-sequencing, we identified the expression profiles of 16 spent culture medium (SCM) collected from embryos at the cleavage on day 3 (D3 cleavage) and blastocyst stages on day 5 (D5 blastocyst) during IVF cycles. Differentially expressed miRNAs (DEmiRNAs) were then identified, and microRNA (miRNA)-messenger RNA (mRNA) interaction networks were constructed. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) confirmation and validation in the Gene Expression Omnibus (GEO) database were performed.
resultsCompared with the pregnant group, 29 DEmiRNAs were detected in the non-pregnant group at D3 cleavage, and 26 were detected in the non-pregnant group at D5 blastocyst. Among them, a total of six known miRNAs, including hsa-miR-199a-3p>hsa-miR-199b-3p, hsa-miR-199a-5p, hsa-miR-379-5p, hsa-miR-432-5p, hsa-miR-99a-5p, and hsa-miR-483-5p, were identified. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these target genes of DEmiRNAs were associated with various biological processes (BPs). The results of validation in qRT-PCR and the GEO database suggested the reliability of our RNA-sequencing results.
conclusionsIn conclusion, we identified three miRNAs, including hsa-miR-199a-5p, hsa-miR-483-5p, and hsa-miR-432-5p, which may serve as biomarkers for embryo quality during IVF cycles.
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