ArticleFrontiers in chemistry2021
Europium Fluorescent Nanoparticles-Based Multiplex Lateral Flow Immunoassay for Simultaneous Detection of Three Antibiotic Families Residue.
Article in Frontiers in chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Rapid Detection of Tulathromycin in Milk with Quantum Dot-Based Fluorescence-Linked Immunosorbent Assay.Foods (Basel, Switzerland) · 2026Article
- Rapid Detection of Tetracycline Residues in Milk Using Colorimetric Sensor Based on Polymethylmethacrylate (PMMA) and Polystyrene (PS) Polymers.Journal of analytical methods in chemistry · 2026Article
- Enhanced immunochromatographic assay of tetracycline using intrinsic peroxidase-like activity of gold nanoparticles.Analytical and bioanalytical chemistry · 2025Article
- Immunoglobulin Y-Based Lateral Flow Immunoassay Strip Test for Detecting Ciprofloxacin Antibiotic in Raw Pork Samples.Foods (Basel, Switzerland) · 2025Article
- Lateral flow assays: Progress and evolution of recent trends in point-of-care applications.Materials today. Bio · 2024Review
- Article
- A Fluorescent Immunochromatography Test Strip for the Rapid Identification of SVV and FMDV.Transboundary and emerging diseases · 2024Article
- Article
- Europium Nanoparticles-Based Fluorescence Immunochromatographic Detection of Three Abused Drugs in Hair.Toxics · 2023Article
- Quantum Dot-Based Lateral Flow Immunoassay as Point-of-Care Testing for Infectious Diseases: A Narrative Review of Its Principle and Performance.Diagnostics (Basel, Switzerland) · 2022Review
- Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process.Frontiers in bioengineering and biotechnology · 2022Review
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fluorescent immunoassay based on europium nanoparticles (EuNPs-FIA) was developed for the simultaneous detection of antibiotic residues, solving the problems of single target detection and low sensitivity of traditional immunoassay methods. In the EuNPs-FIA, EuNPs were used as indictive probes by binding to anti-tetracyclines monoclonal antibodies (anti-TCs mAb), anti-sulphonamides monoclonal antibodies (anti-SAs mAb) and anti-fluoroquinolones monoclonal antibodies (anti-FQs mAb), respectively. Different artificial antigens were assigned to different regions of the nitrocellulose membrane as capture reagents. The EuNPs-FIA allowed for the simultaneous detection of three classes of antibiotics (tetracyclines, fluoroquinolones and sulphonamides) within 15 min. It enabled both the qualitative determination with the naked eye under UV light and the quantitative detection of target antibiotics by scanning the fluorescence intensity of the detection probes on the corresponding detection lines. For qualitative analysis, the cut-off values for tetracyclines (TCs), fluoroquinolones (FQs) and sulphonamides (SAs) were 3.2 ng/ml, 2.4 ng/ml and 4.0 ng/ml, respectively, which were much lower than the maximum residue limit in food. For quantitative analysis, these ranged from 0.06 to 6.85 ng/ml for TCs, 0.03-5.14 ng/ml for FQs, and 0.04-4.40 ng/ml for SAs. The linear correlation coefficients were higher than 0.97. The mean spiked recoveries ranged from 92.1 to 106.2% with relative standard deviations less than 8.75%. Among them, the three monoclonal antibodies could recognize four types of TCs, seven types of FQs and 13 types of SAs, respectively, and the detection range could cover 24 antibiotic residues with different structural formulations. The results of the detection of antibiotic residues in real samples using this method were highly correlated with those of high performance liquid chromatography (
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