Simultaneous Effects of High Intensity Interval Training and Human Amniotic Membrane Scaffold on Rat Tibialis Anterior Vascularization and Innervation after Volumetric Muscle Loss Injury.

M R Izadi, A Habibi, Z Khodabandeh, M Nikbakht

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Article in International journal of organ transplantation medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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M R IzadiDepartment of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

A HabibiDepartment of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Z KhodabandehStem Cell Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

M NikbakhtStem Cell Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

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6 · The paper itself

Abstract

backgroundDespite the high regenerative capacity of skeletal muscle, volumetric muscle loss (VML) is an irrecoverable injury. One therapeutic approach is the implantation of engineered biologic scaffolds.

objectiveTo investigate the simultaneous effect of high intensity interval training (HIIT) and the use of decellularized human amniotic membrane (dHAM) scaffolds on vascularization, growth factor, and neurotrophic factor gene expression, and muscle force generation in the tibialis anterior (TA) of rats after VML injury.

methodsVML injury was created in the TA of 24 rats, which were randomly divided into two groups-12 animals with and 12 without the use of a dHAM scaffold. After injury, each group was further divided into two groups of 6 animals each-sedentary and HIIT. Blood vessels were visualized and counted by hematoxylin and eosin staining. The PowerLab converter assay was used to evaluate isometric contraction force. The relative expression of neurotrophic factors and growth factor genes was measured with reverse transcription PCR (RT-PCR).

resultsThe number of blood vessels in the whole regenerating areas showed a significant difference in the dHAM-HIIT and dHAM-sedentary groups compared to the sedentary group without dHAM (p=0.001 and p=0.003, respectively).

conclusionCombined use of dHAM scaffoldsand HIIT would improve the structure of the injured muscle during regeneration after VML by better vascular perfusion. HIIT leads to greater force generation and innervation by modulating neurotrophic factor synthesis in regenerating muscles.

Indexed as

High intensity interval trainingNeurotrophic factorsScaffoldTissue engineeringVascularization

Identifiers

PMID34987731

PMCPMC8717877

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.