Evidence map›Paper›PMID 34987109›Full record

ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2022

Human Astrocytes Exhibit Tumor Microenvironment-, Age-, and Sex-Related Transcriptomic Signatures.

Mitchell C Krawczyk, Jillian R Haney, Lin Pan, Christine Caneda, Rana R Khankan, Samuel D Reyes, Julia W Chang, Marco Morselli, Harry V Vinters, Anthony C Wang and 12 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 48 citations in OpenAlex.

  1. Article
  2. Astrocyte Regulation of Spinal Circuit Function.Journal of neurochemistry · 2026
    Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Article
  8. Astrocytes in aging.Neuron · 2025
    Review
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. β-Adrenergic Signaling Promotes Morphological Maturation of Astrocytes in Female Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 6 institutions in 1 country.

Mitchell C KrawczykDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Jillian R HaneyDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Lin PanDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Christine CanedaDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Rana R KhankanDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Samuel D ReyesDepartment of Neurosurgery, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Julia W ChangDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Marco MorselliDepartment of Molecular, Cell and Developmental Biology, UCLA-DOE Institute for Genomics and Proteomics, Institute for Quantitative and Computational Biosciences - The Collaboratory at University of California, Los Angeles, California, 90024.
Harry V VintersDepartment of Pathology and Lab Medicine (Neuropathology) and Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Anthony C WangDepartment of Neurosurgery, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Inma CobosDepartment of Pathology, Stanford University, Stanford, California, 94305.
Michael J GandalDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.ORCID 0000-0001-5800-5128
Marvin BergsneiderDepartment of Neurosurgery, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Won KimDepartment of Neurosurgery, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Linda M LiauDepartment of Neurosurgery, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
William YongDepartment of Pathology, University of California, Irvine, California, 90095.
Ali JalaliDepartment of Neurosurgery, Baylor College of Medicine, Houston, Texas, 77030.ORCID 0000-0003-0097-6081
Benjamin DeneenDepartment of Neurosurgery, Baylor College of Medicine, Houston, Texas, 77030.
Gerald A GrantDepartment of Neurosurgery, Stanford University, Stanford, California, 94305.
Gary W MathernDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Aria FallahDepartment of Neurosurgery, David Geffen School of Medicine, University of California, Los Angeles, California, 90024.
Ye ZhangDepartment of Psychiatry and Biobehavioral Sciences, Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, 90024 yezhang@ucla.edu.
University of California, Los Angeles · USBaylor College of Medicine · USStanford University · USAllen Institute for Brain Science · USQB3 · USUniversity of California, Irvine · US

Funding

UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
UCLA SPORE in Brain CancerP50CA211015 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI David A. Nathanson · 2017 to 2026
$25.2M
UCLA IDDRC: Translational CoreP50HD103557 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Peyman Golshani, SUMA JACOB · 2020 to 2026
$9.6M
Training Grant in Neurobehavioral GeneticsT32MH073526 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BEARDEN, CARRIE E, GESCHWIND, DANIEL H · 2006 to 2020
$2.4M
Molecular characterization of reactive astrocytes in humansR01NS109025 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHANG, YE · 2018 to 2022
$1.7M
Identification of transcription factors that regulate astrocyte differentiationR00NS089780 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHANG, YE · 2017 to 2019
$745k
Identification of candidate juvenile protective factors in neuron, glia, and vascular cells of human and mouse brainR03AG065772 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHANG, YE · 2020 to 2021
$306k
NCATS NIH HHS UL1 TR001881NCI NIH HHS P50 CA211015NIA NIH HHS R03 AG065772NIMH NIH HHS T32 MH073526NINDS NIH HHS R00 NS089780NINDS NIH HHS R01 NS109025
6 · The paper itself

Abstract

Astrocytes are critical for the development and function of synapses. There are notable species differences between human astrocytes and commonly used animal models. Yet, it is unclear whether astrocytic genes involved in synaptic function are stable or exhibit dynamic changes associated with disease states and age in humans, which is a barrier in understanding human astrocyte biology and its potential involvement in neurologic diseases. To better understand the properties of human astrocytes, we acutely purified astrocytes from the cerebral cortices of over 40 humans across various ages, sexes, and disease states. We performed RNA sequencing to generate transcriptomic profiles of these astrocytes and identified genes associated with these biological variables. We found that human astrocytes in tumor-surrounding regions downregulate genes involved in synaptic function and sensing of signals in the microenvironment, suggesting involvement of peritumor astrocytes in tumor-associated neural circuit dysfunction. In aging, we also found downregulation of synaptic regulators and upregulation of markers of cytokine signaling, while in maturation we identified changes in ionic transport with implications for calcium signaling. In addition, we identified subtle sexual dimorphism in human cortical astrocytes, which has implications for observed sex differences across many neurologic disorders. Overall, genes involved in synaptic function exhibit dynamic changes in the peritumor microenvironment and aging. These data provide powerful new insights into human astrocyte biology in several biologically relevant states that will aid in generating novel testable hypotheses about homeostatic and reactive astrocytes in humans.

Indexed as

AstrocytesTranscriptomeAgingAnimalsFemaleHumansMaleMiceSynapsesTumor Microenvironmentagingastrocyteglioblastomahumanmaturationsex

Identifiers

PMID34987109
PMCPMC8883850
OpenAlexW4220894177

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.