ArticleApplied and environmental microbiology2022
A Sensitive and Rapid Wastewater Test for SARS-COV-2 and Its Use for the Early Detection of a Cluster of Cases in a Remote Community.
Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Wastewater Respiratory Virus Surveillance in Remote Community, Alaska, USA, 2022-2024.Emerging infectious diseases · 2026Article
- Article
- Identification of circulating human papillomavirus types through high-throughput sequencing of Canadian municipal and institutional wastewater samples.Applied and environmental microbiology · 2025Article
- Public health actions in response to pathogen detection in wastewater and the environment: a scoping review.Frontiers in public health · 2025Article
- A Review of Wastewater-Based Epidemiology for the SARS-CoV-2 Virus in Rural, Remote, and Resource-Constrained Settings Internationally: Insights for Implementation, Research, and Policy for First Nations in Canada.International journal of environmental research and public health · 2024Review
- Research Progress on Detection of Pathogens in Medical Wastewater by Electrochemical Biosensors.Molecules (Basel, Switzerland) · 2024Review
- Wastewater surveillance monitoring of SARS-CoV-2 variants of concern and dynamics of transmission and community burden of COVID-19.Emerging microbes & infections · 2023Article
- The lead time and geographical variations of Baidu Search Index in the early warning of COVID-19.Scientific reports · 2023Article
- Structured Ethical Review for Wastewater-Based Testing in Support of Public Health.Environmental science & technology · 2023Article
- COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities.iScience · 2023Article
- Stabilization of SARS-CoV-2 RNA in wastewater via rapid RNA extraction.The Science of the total environment · 2023Article
- Structured Ethical Review for Wastewater-Based Testing.medRxiv : the preprint server for health sciences · 2023Article
- Real-time quantitative reverse transcription polymerase chain reaction detection of SARS-CoV-2 Delta variant in Canadian wastewater.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2023Article
- A rapid, high-throughput, and sensitive PEG-precipitation method for SARS-CoV-2 wastewater surveillance.Water research · 2023Article
- COVID-19 preparedness and response in rural and remote areas: A scoping review.PLOS global public health · 2023Article
- Wastewater-based epidemiology: the crucial role of viral shedding dynamics in small communities.Frontiers in public health · 2023Article
- Wastewater Surveillance-"Messy" Science With Public Health Potential.American journal of public health · 2023Article
- Long-term passive wastewater surveillance of SARS-CoV-2 for seven university dormitories in comparison to municipal surveillance.The Science of the total environment · 2022Article
- The Detection of SARS-CoV2 Antigen in Wastewater Using an Automated Chemiluminescence Enzyme Immunoassay.International journal of environmental research and public health · 2022Article
- Wastewater surveillance in smaller college communities may aid future public health initiatives.PloS one · 2022Article
Corrections and comments
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Authors and funding
17 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Throughout the coronavirus disease 2019 (COVID-19) pandemic, wastewater surveillance has been used to monitor trends in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) prevalence in the community. A major challenge in establishing wastewater surveillance programs, especially in remote areas, is the need for a well-equipped laboratory for sample analysis. Currently, no options exist for rapid, sensitive, mobile, and easy-to-use wastewater tests for SARS-CoV-2. The performance of the GeneXpert system, which offers cartridge-based, rapid molecular clinical testing for SARS-CoV-2 in a portable platform, was evaluated using wastewater as the input. The GeneXpert demonstrated a SARS-CoV-2 limit of detection in wastewater below 32 copies/mL with a sample processing time of less than an hour. Using wastewater samples collected from multiple sites across Canada during February and March 2021, a high overall agreement (97.8%) was observed between the GeneXpert assay and laboratory-developed tests regarding the presence or absence of SARS-CoV-2. Additionally, with the use of centrifugal filters, the detection threshold of the GeneXpert system was improved to <10 copies/mL in wastewater. Finally, to support on-site wastewater surveillance, GeneXpert testing was implemented in Yellowknife, a remote community in Northern Canada, where its use successfully alerted public health authorities to undetected transmission of COVID-19. The identification of SARS-CoV-2 in wastewater triggered clinical testing of recent travelers and identification of new COVID-19 cases/clusters. Taken together, these results suggest that GeneXpert is a viable option for surveillance of SARS-CoV-2 in wastewater in locations that do not have access to established testing laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.