Evidence map›Paper›PMID 34985866›Full record

ArticleACS synthetic biology2022

Engineering a Dynamic Controllable Infectivity Switch in Bacteriophage T7.

Chutikarn Chitboonthavisuk, Chun Huai Luo, Phil Huss, Mikayla Fernholz, Srivatsan Raman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Chutikarn ChitboonthavisukDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
Chun Huai LuoDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
Phil HussDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
Mikayla FernholzDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
Srivatsan RamanDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.ORCID 0000-0003-2461-1589
University of Wisconsin–Madison · US

Funding

Understanding molecular rules governing bacteriophage specificity and virulence by high-throughput mutational and metagenomic scanningR21AI156785 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI RAMAN, SRIVATSAN · 2021 to 2022
$427k
NIAID NIH HHS R21 AI156785
6 · The paper itself

Abstract

Transcriptional repressors play an important role in regulating phage life cycle. Here, we examine how synthetic transcription repressors can be used in bacteriophage T7 to create a dynamic, controllable infectivity switch. We engineered T7 phage by replacing a large region of the early phage genome with different combinations of ligand-responsive promoters and ribosome binding sites (RBS) designed to control the phage RNA polymerase,

Indexed as

BacteriophagesBacteriophage T7DNA-Directed RNA PolymerasesPromoter Regions, GeneticTranscription FactorsDNA-Directed RNA PolymerasesTranscription Factorsengineered bacteriophagesenterobacteria phage T7ligand-responsive repression systempseudolysogenic

Identifiers

PMID34985866
PMCPMC9059553
OpenAlexW4220749760

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.