Evidence map›Paper›PMID 34985749›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression.

Imre Banlaki, François-Xavier Lehr, Henrike Niederholtmeyer

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Imre BanlakiMax Planck Institute for Terrestrial Microbiology, Marburg, Germany.
François-Xavier LehrMax Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Henrike NiederholtmeyerMax Planck Institute for Terrestrial Microbiology, Marburg, Germany. henrike.niederholtmeyer@synmikro.mpi-marburg.mpg.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Engineering simple, artificial models of living cells allows synthetic biologists to study cellular functions under well-controlled conditions. Reconstituting multicellular behaviors with synthetic cell-mimics is still a challenge because it requires efficient communication between individual compartments in large populations. This chapter presents a microfluidic method to produce large quantities of cell-mimics with highly porous, stable, and chemically modifiable polymer membranes that can be programmed on demand with nucleus-like DNA-hydrogel compartments for gene expression. We describe expression of genes encoded in the hydrogel compartment and communication between neighboring cell-mimics through diffusive protein signals.

Indexed as

Artificial CellsMicrofluidicsGene ExpressionPolymersPorosityPolymersArtificial cellsCell-free transcription and translationCommunicationDNA hydrogelDouble emulsionsMicrofluidicsSynthetic multicellular systems

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.