Evidence map›Paper›PMID 34985741›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems.

Conary Meyer, Chuqing Zhou, Zecong Fang, Marjorie L Longo, Tingrui Pan, Cheemeng Tan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Conary MeyerDepartment of Biomedical Engineering, University of California, Davis, CA, USA.ORCID 0000-0002-4007-8385
Chuqing ZhouDepartment of Biomedical Engineering, University of California, Davis, CA, USA.ORCID 0000-0002-0125-0365
Zecong FangDepartment of Biomedical Engineering, University of California, Davis, CA, USA.ORCID 0000-0003-0491-0207
Marjorie L LongoDepartment of Chemical Engineering, University of California, Davis, CA, USA.ORCID 0000-0001-7854-5746
Tingrui PanDepartment of Biomedical Engineering, University of California, Davis, CA, USA.ORCID 0000-0002-8851-0667
Cheemeng TanDepartment of Biomedical Engineering, University of California, Davis, CA, USA. cmtan@ucdavis.edu.ORCID 0000-0003-1049-1192

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI YOUNG, THOMAS MICHAEL · 1987 to 2021
$50.1M
Engineering and dissecting the synthetic non-dividing-but-active state of hybrid cell-materialsR35GM142788 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Cheemeng Tan · 2021 to 2026
$2.7M
NIEHS NIH HHS P42 ES004699NIGMS NIH HHS R35 GM142788
6 · The paper itself

Abstract

Cell-free protein synthesis can enable the combinatorial screening of many different components and concentrations. However, manual pipetting methods are unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.

Indexed as

Microfluidic Analytical TechniquesMicrofluidicsBiological AssayCell-Free SystemHigh-Throughput Screening AssaysAutomationCell-free protein synthesisDroplet printingHigh throughputLow-volume liquid handlingMicrofluidic adaptive printingProtein screening

Identifiers

PMID34985741
PMCPMC10228547

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.