Evidence map›Paper›PMID 34985737›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Preparation and Screening of Cell-Free Extract from Nongrowing Escherichia coli A19 Cells.

Florian Hiering, Jurek Failmezger, Martin Siemann-Herzberg

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Florian HieringInstitute of Biochemical Engineering, Universität Stuttgart, Stuttgart, Germany.
Jurek FailmezgerInstitute of Biochemical Engineering, Universität Stuttgart, Stuttgart, Germany.
Martin Siemann-HerzbergInstitute of Biochemical Engineering, Universität Stuttgart, Stuttgart, Germany. martin.siemann-herzberg@ibvt.uni-stuttgart.de.
University of Stuttgart · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free extracts have been researched and continuously streamlined for around 50 years. It is believed that these extracts work best when routinely obtained from exponentially growing cells to capture the most active translation system. Here we report on an active cell-free extract derived from E. coli A19 that was harvested under nongrowing, stressed conditions. Although this process is based on the conventional routine process for the production of S30-extracts, our process is less labor intensive and reduces variability between extracts.

Indexed as

Escherichia coliEscherichia coli ProteinsCell ExtractsCell-Free SystemProtein BiosynthesisCell ExtractsEscherichia coli ProteinsCell extractCell-free protein synthesisGrowing conditionsIn vitro transcription and translationRibosomeS30 lysateStationary phaseStressSynthetic biologyTX-TL

Identifiers

PMID34985737
OpenAlexW4206240823

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.