Evidence map›Paper›PMID 34985692›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Indirect Methods To Measure Unfolded Proteins In Living Cells Using Fluorescent Proteins.

Elisabet Navarro-Tapia, Roberto Pérez-Torrado

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Elisabet Navarro-TapiaInstituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC, Paterna, Spain.
Roberto Pérez-TorradoInstituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC, Paterna, Spain. rober@iata.csic.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the study of the unfolded protein response pathway, it is essential to determine the amount of unfolded proteins that the cell is accumulating. Besides being essential it is one of the most challenging technique because of the difficulty to detect unfolded proteins without producing protein denaturation with the method itself. Thus, indirect methods became very useful as the use of fluorescent proteins. In this chapter, we present some of the most used methods to indirectly measure protein folding in living cells using fluorescent proteins.

Indexed as

Endoplasmic ReticulumEndoplasmic Reticulum StressProtein FoldingProteinsUnfolded Protein ResponseProteinsEndoplasmic reticulum stressFluorescent proteinsIndirect methodUnfolded protein response

Identifiers

PMID34985692

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.