Evidence map›Paper›PMID 34985660›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF).

Claudia Alteri, Lorenzo Piermatteo, Francesca Ceccherini Silberstein, Valentina Svicher, Carlo Federico Perno

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Claudia AlteriDepartment of Oncology and Hemato-Oncology, University of Milan, Milan, Italy.
Lorenzo PiermatteoDepartment of Experimental Medicine, University of Rome, Rome, Italy.
Francesca Ceccherini SilbersteinDepartment of Experimental Medicine, University of Rome, Rome, Italy.
Valentina SvicherDepartment of Experimental Medicine, University of Rome, Rome, Italy.
Carlo Federico PernoSaint Camillus International University of Health and Medical Sciences, Rome, Italy. carlofederico.perno@unicamillus.org.
Saint Camillus International University of Health and Medical Sciences · ITUniversity of Milan · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of monocyte subsets and macrophages and their role in HIV pathogenesis, and as long-lived HIV reservoir. Thus, sensitive and specific techniques are needed to measure the impact of these cells in the establishment of the "hard-core" reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte-macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.

Indexed as

Granulocyte-Macrophage Colony-Stimulating FactorHIV InfectionsMacrophagesMonocytesCells, CulturedHIV-1HumansGranulocyte-Macrophage Colony-Stimulating FactorCell cultureGM-CSFHIVMDMMonocytes/macrophagesReservoir

Identifiers

PMID34985660
OpenAlexW4205312233

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.