Evidence map›Paper›PMID 34985327›Full record

ArticleMicrobiology spectrum2022

Quantitative Monitoring of Mycelial Growth of Aspergillus fumigatus in Liquid Culture by Optical Density.

Ken Miyazawa, Takashi Umeyama, Yasutaka Hoshino, Keietsu Abe, Yoshitsugu Miyazaki

Abstract read
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Microorganisms · 2026
    Article
  3. Functional and molecular characterization ofJournal of medical microbiology · 2026
    Article
  4. Role of Iron Availability in ModulatingJournal of fungi (Basel, Switzerland) · 2026
    Article
  5. The Utilization ofMicroorganisms · 2024
    Article
  6. Article
  7. Article
  8. Assessing thermal adaptation of a global sample ofFrontiers in public health · 2023
    Article
  9. Article
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ken MiyazawaDepartment of Fungal Infection, National Institute of Infectious Diseasesgrid.410795.e, Tokyo, Japan.ORCID 0000-0001-9211-1020
Takashi UmeyamaDepartment of Fungal Infection, National Institute of Infectious Diseasesgrid.410795.e, Tokyo, Japan.ORCID 0000-0002-6635-4288
Yasutaka HoshinoDepartment of Fungal Infection, National Institute of Infectious Diseasesgrid.410795.e, Tokyo, Japan.
Keietsu AbeLaboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.ORCID 0000-0001-5344-3794
Yoshitsugu MiyazakiDepartment of Fungal Infection, National Institute of Infectious Diseasesgrid.410795.e, Tokyo, Japan.ORCID 0000-0001-8942-0869

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Filamentous fungi form multicellular hyphae, which generally form pellets in liquid shake cultures, during the vegetative growth stage. Because of these characteristics, growth-monitoring methods commonly used in bacteria and yeast have not been applied to filamentous fungi. We have recently revealed that the cell wall polysaccharide α-1,3-glucan and extracellular polysaccharide galactosaminogalactan (GAG) contribute to hyphal aggregation in Aspergillus oryzae. Here, we tested whether Aspergillus fumigatus shows dispersed growth in liquid media that can be quantitatively monitored, similar to that of yeasts. We constructed a double disruptant mutant of both the primary α-1,3-glucan synthase gene

Indexed as

Antifungal AgentsAspergillus fumigatusCell WallCulture MediaFungal ProteinsGlucansGlucosyltransferasesHyphaeMyceliumSpectrophotometryAntifungal AgentsCulture MediaFungal ProteinsGlucansglucan synthaseGlucosyltransferases3-glucanAspergillus fumigatusfilamentous fungigalactosaminogalactanhyphal aggregationoptical densityα-1

Identifiers

PMID34985327
PMCPMC8729762

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.