Evidence map›Paper›PMID 34984599›Full record

ArticleJournal of assisted reproduction and genetics2022

Effect of cumulin and super-GDF9 in standard and biphasic mouse IVM.

Nazli Akin, Dulama Richani, Xiuhua Liao, Yiqing Zhao, Anamaria-Cristina Herta, Katy Billooye, William A Stocker, David G Mottershead, Craig A Harrison, Johan Smitz and 2 more

Registry-linked trialOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07197255 (Transcriptomic Profiling of Cumulus Cells From CAPA-IVM Cycles in Women With PCOS), which is not on this map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07197255 recruitingnot on this mapstarted 2025, after this paper: background citation

Transcriptomic Profiling of Cumulus Cells From CAPA-IVM Cycles in Women With PCOS: A Prospective Pilot Study

TypeobservationalSponsorMỹ Đức HospitalRan2025 to 2026Enrolled10ConditionsPCOS (Polycystic Ovary Syndrome), Cumulus Cell, Capacitation IVM, Transcriptomic Profiling
3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. In vitro maturation (IVM) of human immature oocytes: is it still relevant?Reproductive biology and endocrinology : RB&E · 2023
    Review
  4. Article
  5. Article
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 5 countries.

Nazli AkinFollicle Biology Laboratory, Vrije Universiteit Brussel (VUB), 1090, Brussels, Belgium.
Dulama RichaniFertility & Research Centre, School of Women's and Children's Health, University of New South Wales Sydney, Sydney, NSW, 2052, Australia. d.richani@unsw.edu.au.ORCID http://orcid.org/0000-0003-1886-0423
Xiuhua LiaoFertility & Research Centre, School of Women's and Children's Health, University of New South Wales Sydney, Sydney, NSW, 2052, Australia.
Yiqing ZhaoFertility & Research Centre, School of Women's and Children's Health, University of New South Wales Sydney, Sydney, NSW, 2052, Australia.
Anamaria-Cristina HertaFollicle Biology Laboratory, Vrije Universiteit Brussel (VUB), 1090, Brussels, Belgium.
Katy BillooyeFollicle Biology Laboratory, Vrije Universiteit Brussel (VUB), 1090, Brussels, Belgium.
William A StockerDepartment of Physiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, 3800, Australia.
David G MottersheadSchool of Pharmacy and Bioengineering, Keele University, Newcastle-under-Lyme, UK.
Craig A HarrisonDepartment of Physiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, 3800, Australia.
Johan SmitzFollicle Biology Laboratory, Vrije Universiteit Brussel (VUB), 1090, Brussels, Belgium.
Ellen AnckaertFollicle Biology Laboratory, Vrije Universiteit Brussel (VUB), 1090, Brussels, Belgium.
Robert B GilchristFertility & Research Centre, School of Women's and Children's Health, University of New South Wales Sydney, Sydney, NSW, 2052, Australia.
Vrije Universiteit Brussel · BEUNSW Sydney · AUAustralian Regenerative Medicine Institute · AUKeele University · GB

Funding

Flanders FWO The Excellence of Science G0F3118NNational Health and Medical Research Council APP1023210National Health and Medical Research Council APP1062762National Health and Medical Research Council APP1076004National Health and Medical Research Council APP1117538National Health and Medical Research Council APP1121504
6 · The paper itself

Abstract

purposeIn vitro maturation (IVM) is a technology that generates mature oocytes following culture of immature cumulus-oocyte complexes (COC) in vitro. IVM is characterized by minimal patient stimulation, making it attractive for certain patient groups. Recently, a biphasic IVM system, capacitation (CAPA)-IVM, has shown improved clinical outcomes relative to standard IVM; however, it remains less efficient than IVF. This study assessed whether supplementation of CAPA-IVM culture media with the novel TGFβ superfamily proteins cumulin and super-GDF9 improves subsequent mouse embryo development.

methodsImmature mouse COCs were cultured by standard IVM or biphasic IVM ± cumulin or super-GDF9.

resultsBoth cumulin and super-GDF9 in standard IVM significantly improved day-6 blastocyst rate (53.9% control, 73.6% cumulin, 70.4% super-GDF9; p = 0.006; n = 382-406 oocytes). Cumulin or super-GDF9 in CAPA-IVM did not alter embryo yield or blastocyst cell allocation in an unstimulated model. Moreover, cumulin did not alter these outcomes in a mild PMSG stimulation model. Cumulin in CAPA-IVM significantly increased cumulus cell expression of cumulus expansion genes (Ptgs2, Ptx3, Adamts1, Gfat2) and decreased Lhr expression relative to control. However, cumulin-induced mRNA expression of cumulus cell (Ptgs2, Ptx3) and oocyte genes (Gdf9, Bmp15, Oct4, Stella) in CAPA-IVM remained significantly lower than that of in vivo matured cells.

conclusionCumulin did not provide an additional beneficial effect in biphasic IVM in terms of blastocyst yield and cell allocation; however in standard IVM, cumulin and super-GDF9 significantly improve oocyte developmental competence.

Indexed as

AnimalsCumulus CellsDisease Models, AnimalGrowth Differentiation Factor 9In Vitro Oocyte Maturation TechniquesMiceMice, Inbred C57BLOogenesisGdf9 protein, mouseGrowth Differentiation Factor 9

Identifiers

PMID34984599
PMCPMC8866628
OpenAlexW4206049587

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.