Evidence map›Paper›PMID 34984589›Full record

ArticleNeurochemical research2022

EGF Downregulates Presynaptic Maturation and Suppresses Synapse Formation In Vitro and In Vivo.

Nobuyuki Takei, Daisaku Yokomaku, Takaho Yamada, Tadasato Nagano, Akiyoshi Kakita, Hisaaki Namba, Tatsuo Ushiki, Hitoshi Takahashi, Hiroyuki Nawa

Abstract read
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In one paragraph

Article in Neurochemical research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Nobuyuki Takei *Department of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan. nobtak@bri.niigata-u.ac.jp.ORCID http://orcid.org/0000-0002-6122-939X
Daisaku Yokomaku *Department of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan.
Takaho YamadaDivision of Microscopic Anatomy, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
Tadasato NaganoDepartment of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan.
Akiyoshi KakitaDepartment of Pathology, Brain Research Institute, Niigata University, Niigata, Japan.
Hisaaki NambaDepartment of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan.
Tatsuo UshikiDivision of Microscopic Anatomy, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
Hitoshi TakahashiDepartment of Pathology, Brain Research Institute, Niigata University, Niigata, Japan.
Hiroyuki NawaDepartment of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan.
Niigata University · JPWakayama Medical University · JPUniversity of Niigata Prefecture · JP

Funding

Japan Society for the Promotion of Science 16K07053Japan Society for the Promotion of Science 18H05429
6 · The paper itself

Abstract

Neuronal differentiation, maturation, and synapse formation are regulated by various growth factors. Here we show that epidermal growth factor (EGF) negatively regulates presynaptic maturation and synapse formation. In cortical neurons, EGF maintained axon elongation and reduced the sizes of growth cones in culture. Furthermore, EGF decreased the levels of presynaptic molecules and number of presynaptic puncta, suggesting that EGF inhibits neuronal maturation. The reduction of synaptic sites is confirmed by the decreased frequencies of miniature EPSCs. In vivo analysis revealed that while peripherally administrated EGF decreased the levels of presynaptic molecules and numbers of synaptophysin-positive puncta in the prefrontal cortices of neonatal rats, EGF receptor inhibitors upregulated these indexes, suggesting that endogenous EGF receptor ligands suppress presynaptic maturation. Electron microscopy further revealed that EGF decreased the numbers, but not the sizes, of synaptic structures in vivo. These findings suggest that endogenous EGF and/or other EGF receptor ligands negatively modulates presynaptic maturation and synapse formation.

Indexed as

Epidermal Growth FactorSynapsesAnimalsAxonsCells, CulturedNeurogenesisNeuronsRatsEpidermal Growth FactorCortical neuronsEGFGrowth coneIn vivoPresynapseSynapse formation

Identifiers

PMID34984589
OpenAlexW4206095656

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.