Evidence map›Paper›PMID 34984553›Full record

ArticleCellular and molecular life sciences : CMLS2022

Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane.

Gaëlle Houthaeve, Gerardo García-Díaz Barriga, Stephan Stremersch, Herlinde De Keersmaecker, Juan Fraire, Jo Vandesompele, Pieter Mestdagh, Stefaan De Smedt, Kevin Braeckmans, Winnok H De Vos

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Gaëlle HouthaeveLaboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2610, Antwerp, Belgium.
Gerardo García-Díaz BarrigaLaboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2610, Antwerp, Belgium.
Stephan StremerschLaboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, 9000, Ghent, Belgium.
Herlinde De KeersmaeckerLaboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, 9000, Ghent, Belgium.
Juan FraireLaboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, 9000, Ghent, Belgium.
Jo VandesompeleCenter for Medical Genetics, Ghent University, 9000, Ghent, Belgium.
Pieter MestdaghCenter for Medical Genetics, Ghent University, 9000, Ghent, Belgium.
Stefaan De SmedtLaboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, 9000, Ghent, Belgium.
Kevin Braeckmans *Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, 9000, Ghent, Belgium.
Winnok H De Vos *Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2610, Antwerp, Belgium. Winnok.DeVos@UAntwerpen.be.ORCID http://orcid.org/0000-0003-0960-6781
University College Ghent · BEUniversity of Antwerp · BEGhent University · BEGhent University Hospital · BE

Funding

Fonds Wetenschappelijk Onderzoek 1152918NFonds Wetenschappelijk Onderzoek FWO 1210120NFonds Wetenschappelijk Onderzoek G005819NFonds Wetenschappelijk Onderzoek I003420NFonds Wetenschappelijk Onderzoek IRI I000321NUniversiteit Antwerpen BOF IMARKUniversiteit Antwerpen µNEURO
6 · The paper itself

Abstract

Vapor nanobubble (VNB) photoporation is a physical method for intracellular delivery that has gained significant interest in the past decade. It has successfully been used to introduce molecular cargo of diverse nature into different cell types with high throughput and minimal cytotoxicity. For translational purposes, it is important to understand whether and how photoporation affects cell homeostasis. To obtain a comprehensive view on the transcriptional rewiring that takes place after VNB photoporation, we performed a longitudinal shotgun RNA-sequencing experiment. Six hours after photoporation, we found a marked upregulation of LMNA transcripts as well as their protein products, the A-type lamins. At the same time point, we observed a significant increase in several heterochromatin marks, suggesting a global stiffening of the nucleus. These molecular features vanished 24 h after photoporation. Since VNB-induced chromatin condensation was prolonged in LMNA knockout cells, A-type lamins may be required for restoring the nucleus to its original state. Selective depletion of A-type lamins reduced cell viability after VNB photoporation, while pharmacological stimulation of LMNA transcription increased the percentage of successfully transfected cells that survived after photoporation. Therefore, our results suggest that cells respond to VNB photoporation by temporary upregulation of A-type lamins to facilitate their recovery.

Indexed as

Cell Membrane PermeabilityCell MembraneCell NucleusGene Expression ProfilingHeLa CellsHumansLamin Type ALightMicrotubulesNanoparticlesPolymerizationProtein BiosynthesisTemperatureTranscription, GeneticTranscriptomeUp-RegulationLamin Type AA-type laminsChromatinGold nanoparticlesPhotoporationPlasma membraneVapor nanobubbles

Identifiers

PMID34984553
PMCPMC8727414
OpenAlexW4205870500

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.