Evidence map›Paper›PMID 34983581›Full record

ArticleJournal of translational medicine2022

microRNA-155-3p delivered by M2 macrophages-derived exosomes enhances the progression of medulloblastoma through regulation of WDR82.

Li Song, Bin Luan, Qingrong Xu, Ruihe Shi, Xiufang Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Li SongDepartment of Pediatric Respiratory Medicine, The 3rd Affiliated Hospital of Zhengzhou University, No. 7, Kangfuqian street, Zhengzhou, 450052, Henan, China.
Bin LuanDepartment of Pediatric Respiratory Medicine, The 3rd Affiliated Hospital of Zhengzhou University, No. 7, Kangfuqian street, Zhengzhou, 450052, Henan, China. Luanbin03969@163.com.
Qingrong XuDepartment of Pediatric Respiratory Medicine, The 3rd Affiliated Hospital of Zhengzhou University, No. 7, Kangfuqian street, Zhengzhou, 450052, Henan, China.
Ruihe ShiDepartment of Pediatric Respiratory Medicine, The 3rd Affiliated Hospital of Zhengzhou University, No. 7, Kangfuqian street, Zhengzhou, 450052, Henan, China.
Xiufang WangDepartment of Pediatric Respiratory Medicine, The 3rd Affiliated Hospital of Zhengzhou University, No. 7, Kangfuqian street, Zhengzhou, 450052, Henan, China.
Zhengzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveExosomes, membranous nanovesicles, naturally bringing proteins, mRNAs, and microRNAs (miRNAs), play crucial roles in tumor pathogenesis. This study was to investigate the role of miR-155-3p from M2 macrophages-derived exosomes (M2-Exo) in promoting medulloblastoma (MB) progression by mediating WD repeat domain 82 (WDR82).

methodsmiR-155-3p expression was detected by RT-qPCR. The relationship of miR-155-3p with clinicopathological features of MB patients was analyzed. M2-Exo were isolated and identified by TEM, NTA and Western blot. CCK-8 assay, colony formation assay, flow cytometry, wound healing assay, and Transwell assay were performed to explore the role of miR-155-3p-enriched M2-Exo on the progression of MB cells. Luciferase assay were used to identify the relationship between miR-155-3p and WDR82. The effect of miR-155-3p-enriched M2-Exo on tumorigenesis of MB was confirmed by the xenograft nude mice model.

resultsmiR-155-3p was up-regulated in MB tissues of patients and MB cell lines. High miR-155-3p expression was correlated with the pathological type and molecular subtype classification of MB patients. WDR82 was a direct target of miR-155-3p. miR-155-3p was packaged into M2-Exo. miR-155-3p-enriched M2-Exo promoted the progression of Daoy cells. miR-155-3p-enriched M2-Exo promoted in vivo tumorigenesis.

conclusionThe study highlights that miR-155-3p-loaded M2-Exo enhances the growth of MB cells via down-regulating WDR82, which might provide a deep insight into MB mechanism.

Indexed as

Cerebellar NeoplasmsExosomesMedulloblastomaMicroRNAsAnimalsCell Line, TumorCell ProliferationChromosomal Proteins, Non-HistoneGene Expression Regulation, NeoplasticHumansMacrophagesMiceMice, NudeChromosomal Proteins, Non-HistoneMicroRNAsMIRN155 microRNA, humanMirn155 microRNA, mouseWdr82 protein, mouseApoptosisExosomesInvasionM2 macrophagesMedulloblastomaMicroRNA-155-3pMigrationProliferationWD repeat domain 82

Identifiers

PMID34983581
PMCPMC8728908
OpenAlexW4205180335

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.