ArticleMicrobial cell factories2022
Dual regulation of lipid droplet-triacylglycerol metabolism and ERG9 expression for improved β-carotene production in Saccharomyces cerevisiae.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 62 citations in OpenAlex.
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- Advances in Metabolic Engineering for the Accumulation of Astaxanthin Biosynthesis.Molecular biotechnology · 2025Review
- Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts.Frontiers in plant science · 2025Article
- Overexpression of tRNA mFrontiers in microbiology · 2025Article
- PunctaFinder: An algorithm for automated spot detection in fluorescence microscopy images.Molecular biology of the cell · 2024Article
- Combined metabolic engineering and lipid droplets degradation to increase vitamin A production in Saccharomyces cerevisiae.Microbial cell factories · 2024Article
- Screening of broad-host expression promoters for shuttle expression vectors in non-conventional yeasts and bacteria.Microbial cell factories · 2024Article
- Recent advances in lycopene and germacrene a biosynthesis and their role as antineoplastic drugs.World journal of microbiology & biotechnology · 2024Review
- Induction of point and structural mutations in engineered yeast Saccharomyces cerevisiae improve carotenoid production.World journal of microbiology & biotechnology · 2024Article
- Development of a metabolic engineering technology to simultaneously suppress the expression of multiple genes in yeast and application in carotenoid production.World journal of microbiology & biotechnology · 2024Article
- Transcending membrane barriers: advances in membrane engineering to enhance the production capacity of microbial cell factories.Microbial cell factories · 2024Review
- Strategies for the efficient biosynthesis of β-carotene through microbial fermentation.World journal of microbiology & biotechnology · 2024Review
- Multi-modular metabolic engineering and efflux engineering for enhanced lycopene production in recombinant Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2024Article
- Multidimensional Optimization ofBiodesign research · 2024Article
- Advances in the Discovery and Engineering of Gene Targets for Carotenoid Biosynthesis in Recombinant Strains.Biomolecules · 2023Review
- Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene inSynthetic and systems biotechnology · 2023Article
- Enhanced β-carotene production in Yarrowia lipolytica through the metabolic and fermentation engineering.Journal of industrial microbiology & biotechnology · 2023Article
- Peroxisome Proliferator-Activated ReceptorPPAR research · 2023Article
- Compartmentalization engineering of yeasts to overcome precursor limitations and cytotoxicity in terpenoid production.Frontiers in bioengineering and biotechnology · 2023Review
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe limitation of storage space, product cytotoxicity and the competition for precursor are the major challenges for efficiently overproducing carotenoid in engineered non-carotenogenic microorganisms. In this work, to improve β-carotene accumulation in Saccharomyces cerevisiae, a strategy that simultaneous increases cell storage capability and strengthens metabolic flux to carotenoid pathway was developed using exogenous oleic acid (OA) combined with metabolic engineering approaches.
resultsThe direct separation of lipid droplets (LDs), quantitative analysis and genes disruption trial indicated that LDs are major storage locations of β-carotene in S. cerevisiae. However, due to the competition for precursor between β-carotene and LDs-triacylglycerol biosynthesis, enlarging storage space by engineering LDs related genes has minor promotion on β-carotene accumulation. Adding 2 mM OA significantly improved LDs-triacylglycerol metabolism and resulted in 36.4% increase in β-carotene content. The transcriptome analysis was adopted to mine OA-repressible promoters and IZH1 promoter was used to replace native ERG9 promoter to dynamically down-regulate ERG9 expression, which diverted the metabolic flux to β-carotene pathway and achieved additional 31.7% increase in β-carotene content without adversely affecting cell growth. By inducing an extra constitutive β-carotene synthesis pathway for further conversion precursor farnesol to β-carotene, the final strain produced 11.4 mg/g DCW and 142 mg/L of β-carotene, which is 107.3% and 49.5% increase respectively over the parent strain.
conclusionsThis strategy can be applied in the overproduction of other heterogeneous FPP-derived hydrophobic compounds with similar synthesis and storage mechanisms in S. cerevisiae.
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