Evidence map›Paper›PMID 34983299›Full record

ArticleBioengineered2022

Implementation of novel boolean logic gates for IMPLICATION and XOR functions using riboregulators.

Chaoxin Chen, Qi Wu, Qingying Ke, Ting Wang, Yifan Zhang, Feiwen Wei, Xiaolong Wang, Guanglei Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Fluorescence-Based Multimodal DNA Logic Gates.Nanomaterials (Basel, Switzerland) · 2024
    Article
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chaoxin ChenThe iGEM Laboratory of OUC-China, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Qi WuThe iGEM Laboratory of OUC-China, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Qingying KeThe iGEM Laboratory of OUC-China, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Ting WangThe iGEM Laboratory of OUC-China, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Yifan ZhangThe iGEM Laboratory of OUC-China, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Feiwen WeiThe iGEM Laboratory of OUC-China, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Xiaolong WangThe iGEM Laboratory of OUC-China, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Guanglei LiuThe iGEM Laboratory of OUC-China, College of Marine Life Sciences, Ocean University of China, Qingdao, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To date, several different types of synthetic genetic switches, including riboregulators, riboswitches, and toehold switches, have been developed to construct AND, OR, NOT, NAND, NOR, and NOT IMPLICATION (NIMP) gates. The logic gate can integrate multiple input signals following a set of algorithms and generate a response only if strictly defined conditions are met. However, there are still some logic gates that have not been implemented but are necessary to build complex genetic circuits. Here, based on the toehold switches and three-way-junction (3WJ) repressors, we designed two novel biological Boolean logic gates of IMPLICATION (IMP) and XOR. Subsequently, the outputs of these two logic gates were characterized by fluorescence analysis, indicating that they can achieve the truth tables of logical gates. Furthermore, the fluorescence intensity under the logical TRUE condition was significantly higher than under the logical FALSE condition, suggesting the high dynamic range of the ON/OFF ratios. Because of the programmability of synthetic RNA switches, the constructed RNA logic gates could serve as elementary units to build a versatile and powerful platform for translational regulation and RNA-based biological computation.

Indexed as

AlgorithmsComputational BiologyComputers, MolecularFluorescenceGene Regulatory NetworksRNASynthetic BiologyRNABiological boolean logic gatesimplication (IMP)riboregulatorRNA regulatory elementssynthetic RNA switchesXOR

Identifiers

PMID34983299
PMCPMC8805959

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.