Evidence map›Paper›PMID 34981333›Full record

ArticleMolecular biology reports2022

EMT in salivary gland tumors: the expression of microRNAs miR-155 and miR-200c is associated with clinical-pathological parameters.

Leandra Ernst Kerche, Elen Alves de Sousa, Cristiane Helena Squarize, Katia Klug Oliveira, Fabio Albuquerque Marchi, Bárbara Beltrame Bettim, Luiz Paulo Kowalski, Fernando Augusto Soares, Silvia Vanessa Lourenço, Cláudia Malheiros Coutinho-Camillo

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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 2 pooled it
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
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  10. The Therapeutic Landscape of Salivary Gland Malignancies-Where Are We Now?International journal of molecular sciences · 2022
    Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Leandra Ernst KercheInternational Research Center, A.C.Camargo Cancer Center, Centro Internacional de Pesquisa, Rua Taguá, 440-Primeiro andar, São Paulo, SP, Brazil.
Elen Alves de SousaInternational Research Center, A.C.Camargo Cancer Center, Centro Internacional de Pesquisa, Rua Taguá, 440-Primeiro andar, São Paulo, SP, Brazil.
Cristiane Helena SquarizeLaboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
Katia Klug OliveiraInternational Research Center, A.C.Camargo Cancer Center, Centro Internacional de Pesquisa, Rua Taguá, 440-Primeiro andar, São Paulo, SP, Brazil.
Fabio Albuquerque MarchiInternational Research Center, A.C.Camargo Cancer Center, Centro Internacional de Pesquisa, Rua Taguá, 440-Primeiro andar, São Paulo, SP, Brazil.
Bárbara Beltrame BettimInternational Research Center, A.C.Camargo Cancer Center, Centro Internacional de Pesquisa, Rua Taguá, 440-Primeiro andar, São Paulo, SP, Brazil.
Luiz Paulo KowalskiDepartment of Head and Neck Surgery and Otorhinolaryngology, A.C.Camargo Cancer Center, São Paulo, SP, Brazil.
Fernando Augusto SoaresPathology Division, Rede D'Or Hospitals Network, São Paulo, SP, Brazil.
Silvia Vanessa LourençoDepartment of General Pathology, Dental School, University of São Paulo, São Paulo, SP, Brazil.
Cláudia Malheiros Coutinho-CamilloInternational Research Center, A.C.Camargo Cancer Center, Centro Internacional de Pesquisa, Rua Taguá, 440-Primeiro andar, São Paulo, SP, Brazil. ccamillo@accamargo.org.br.ORCID http://orcid.org/0000-0001-9016-2668
AC Camargo Hospital · BRD’Or Institute for Research and Education · BRUniversidade de São Paulo · BRUniversity of Michigan · US

Funding

CNPq 443243/2014-0FAPESP 11/02051-6
6 · The paper itself

Abstract

backgroundEpithelial to mesenchymal transition promotes cell adhesion loss, enabling invasion and metastasis. MicroRNAs are a class of small non-codifying RNAs that regulate gene expression.

objectivesThe aim of this study was to evaluate the expression of microRNAs that could regulate the expression of EMT factors in salivary gland tumors (SGTs). METHODS AND

resultsThe expression of microRNAs miR-9, miR-34a, miR-101, miR-138, miR-155, and miR-200c-described in the literature to target EMT factors-was evaluated by Real-time RT-PCR (qPCR) in pleomorphic adenoma (PA), mucoepidermoid carcinoma (MEC) and adenoid cystic carcinoma (ACC) samples. Bioinformatics tools were applied to identify miR targets and immunohistochemistry was used to examine the expression of the proteins E-cadherin, Twist, ZEB-1, β-Catenin, and c-Kit. Comparing miR expression among SGT types, we observed increased expression of miR-9, and miR-138 in PAs, and increased miR-155 expression in MECs. Low-grade MECs exhibited increased miR-155 expression (p = 0.032). MECs that generated lymph node metastases had increased miR-200c levels (p = 0.018). MECs tended to have decreased expression of EMT-related proteins when compared to the other SGT types (c-Kit p < 0.001, Twist p = 0.014, and ZEB p = 0.012). Notably, increased c-Kit expression was associated with the presence of perineural infiltration in ACC (p = 0.050).

conclusionsThis study provides evidence of alterations in the expression of EMT-factors regulating miRs, especially of miR-9, miR-138, miR-155, and miR-200c. No significant relationships were found between the expression of these miRs and proteins associated with EMT in SGTs.

Indexed as

MicroRNAsSalivary Gland NeoplasmsCadherinsCell Line, TumorCell MovementEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHumansCadherinsMicroRNAsMIRN138 microRNA, humanMIRN155 microRNA, humanMIRN200 microRNA, humanMIRN92 microRNA, humanEpithelial-mesenchymal transitionImmunohistochemistrymiRNAsqPCRSalivary gland tumors

Identifiers

PMID34981333
OpenAlexW4206413983

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.