ArticleBMC biotechnology2022
Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology.
Article in BMC biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 17 citations in OpenAlex.
- Illuminating spatial dynamics of glutamine metabolism with a sensitive genetically encoded biosensor.RSC chemical biology · 2026Article
- High-yield soluble expression and affinity-tag-free purification of growth hormone receptor antagonist B2036 in Escherichia coli.Bioprocess and biosystems engineering · 2026Article
- Revealing the spatiotemporal dynamics of methionine metabolism with a genetically encoded single-fluorophore biosensor.bioRxiv : the preprint server for biology · 2026Article
- A Simple Method to Produce Recombinant Mammalian Serum Albumins inInternational journal of molecular sciences · 2026Article
- Illuminating spatial dynamics of glutamine metabolism with a sensitive genetically encoded biosensor.bioRxiv : the preprint server for biology · 2026Article
- Cell cycle arrest mitigates the loss of ribonucleic acid content in Saccharomyces pastorianus.World journal of microbiology & biotechnology · 2025Article
- Induction conditions that promote the effect of glycerol on recombinant protein production inBiotechnology reports (Amsterdam, Netherlands) · 2025Article
- A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA.ACS bio & med chem Au · 2025Article
- Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli.Scientific reports · 2024Article
- Increased cytoplasmic expression of PETase enzymes in E. coli.Microbial cell factories · 2024Article
- A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA.bioRxiv : the preprint server for biology · 2024Article
- Response Surface Methodology to Optimize the Expression Efficiency of Recombinant Reteplase.Iranian journal of biotechnology · 2023Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSHuffle is a suitable Escherichia coli (E. coli) strain for high yield cytoplasmic soluble expression of disulfide-bonded proteins such as Insulin due to its oxidative cytoplasmic condition and the ability to correct the arrangement of disulfide bonds. Lispro is an Insulin analog that is conventionally produced in E. coli as inclusion bodies (IBs) with prolonged production time and low recovery. Here in this study, we aimed to optimize cultivation media composition for high cell density fermentation of SHuffle T7 E. coli expressing soluble Lispro proinsulin fused to SUMO tag (SU-INS construct) to obtain high cell density fermentation.
resultsFactors including carbon and nitrogen sources, salts, metal ions, and pH were screened via Plackett-Burman design for their effectiveness on cell dry weight (CDW) as a measure of cell growth. The most significant variables of the screening experiment were Yeast extract and MgCl
conclusionThe proposed media was suitable for high cell density fermentation of E. coli SHuffle T7 and was applicable for high yield soluble expression of Lispro proinsulin.
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