ArticleBMC plant biology2022
Endophytic fungus Pseudodidymocyrtis lobariellae KL27 promotes taxol biosynthesis and accumulation in Taxus chinensis.
Article in BMC plant biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 35 citations in OpenAlex.
- Plant-Microbiome Interactions in Medicinal Plants: A Synergistic Partnership for Biomass Production and Secondary Metabolite Accumulation.Microorganisms · 2026Review
- Physiology, Metabolomics, and Transcriptomics Reveal Effects of AMF andMetabolites · 2026Article
- Harnessing Endophytic Fungi as a Sustainable Source of Novel Anticancer Agents: Opportunities, Challenges, and Future Directions.Molecules (Basel, Switzerland) · 2026Review
- Inspired by taxol: biosynthesis and engineering of hypericin and other phytochemicals in Hypericum.Planta · 2026Review
- Microbial elicitors to metabolic reprogramming: an integrative model of plant-microbe interactions.Frontiers in microbiology · 2026Review
- A fungal endophyte of the medicinal plant,Frontiers in microbiology · 2026Article
- TcJAMYC5 positively regulates paclitaxel biosynthesis in Taxus chinensis var. Mairei.Plant molecular biology · 2025Article
- A new Clonostachys Sp. ZBS49 filamentous fungus with high production of betulinic acid and its inhibitory effect on liver cancer cells.Microbial cell factories · 2025Article
- Multidimensional regulation of transcription factors: decoding the comprehensive signals of plant secondary metabolism.Frontiers in plant science · 2025Review
- Effects and molecular mechanism of endophytic elicitors on the accumulation of secondary metabolites in medicinal plants.Frontiers in microbiology · 2025Review
- Paclitaxel production from endophytic3 Biotech · 2024Article
- Fungal endophytes of Taxus species and regulatory effect of two strains on taxol synthesis.BMC microbiology · 2024Article
- Effects of elicitors from culture filtrate ofFrontiers in plant science · 2024Article
- Enhancement of paclitaxel production by Neopestalotiopsis vitis via optimization of growth conditions.PloS one · 2024Article
- Cultivable Endophyte Resources in Medicinal Plants and Effects on Hosts.Life (Basel, Switzerland) · 2023Review
- Diversity of Endophytic Microbes inMicroorganisms · 2023Article
- Endophyte-inoculated rhizomes ofFrontiers in microbiology · 2023Article
- The determination of the biological function of bacterial pink pigment andFrontiers in microbiology · 2023Article
- Exploring the anticancer activities of novel bioactive compounds derived from endophytic fungi: mechanisms of action, current challenges and future perspectives.American journal of cancer research · 2022Review
- TcMYB29a, an ABA-Responsive R2R3-MYB Transcriptional Factor, Upregulates Taxol Biosynthesis inFrontiers in plant science · 2022Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTaxol from Taxus species is a precious drug used for the treatment of cancer and can effectively inhibit the proliferation of cancer cells. However, the growth of Taxus plants is very slow and the content of taxol is quite low. Therefore, it is of great significance to improve the yield of taxol by modern biotechnology without destroying the wild forest resources. Endophytic fungus which symbiosis with their host plants can promote the growth and secondary metabolism of medicinal plants.
resultsHere, an endophytic fungus KL27 was isolated from T. chinensis, and identified as Pseudodidymocyrtis lobariellae. The fermentation broth of KL27 (KL27-FB) could significantly promote the accumulation of taxol in needles of T. chinensis, reaching 0.361 ± 0.082 mg/g·DW (dry weight) at 7 days after KL27-FB treatment, which is 3.26-fold increase as compared to the control. The RNA-seq and qRT-PCR showed that KL27-FB could significantly increase the expression of key genes involved in the upstream pathway of terpene synthesis (such as DXS and DXR) and those in the taxol biosynthesis pathway (such as GGPPS, TS, T5OH, TAT, T10OH, T14OH, T2OH, TBT, DBAT and PAM), especially at the early stage of the stimulation. Moreover, the activation of jasmonic acid (JA) biosynthesis and JA signal transduction, and its crosstalk with other hormones, such as gibberellin acid (GA), ethylene (ET) and salicylic acid (SA), explained the elevation of most of the differential expressed genes related to taxol biosynthesis pathway. Moreover, TF (transcriptional factor)-encoding genes, including MYBs, ethylene-responsive transcription factors (ERFs) and basic/helix-loop-helix (bHLH), were detected as differential expressed genes after KL27-FB treatment, further suggested that the regulation of hormone signaling on genes of taxol biosynthesis was mediated by TFs.
conclusionsOur results indicated that fermentation broth of endophytic fungus KL27-FB could effectively enhance the accumulation of taxol in T. chinensis needles by regulating the phytohormone metabolism and signal transduction and further up-regulating the expression of multiple key genes involved in taxol biosynthesis. This study provides new insight into the regulatory mechanism of how endophytic fungus promotes the production and accumulation of taxol in Taxus sp.
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