ArticlePlant physiology2022
pHBMT1, a BAHD-family monolignol acyltransferase, mediates lignin acylation in poplar.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Simultaneous Overexpression of FERULOYL-CoA 6'-HYDROXYLASE 1 and COUMARIN SYNTHASE Leads to Coumarin-Enriched Lignin and Improved Saccharification in Greenhouse- and Field-Grown Poplar.Plant biotechnology journal · 2026Article
- Functional characterization of Acyl-CoA Synthase 1 in tomato branch angle development.BMC plant biology · 2026Article
- Identification of the BAHD gene family in Korla fragrant pear and functional characterization of PsBAHD6 in resistance to pear Valsa canker.BMC plant biology · 2026Article
- Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family inPlants (Basel, Switzerland) · 2025Article
- Pd/C promotes C-H bond activation and oxidation of p-hydroxybenzoate during hydrogenolysis of poplar.Nature communications · 2025Article
- Article
- Synthetic-biology approach for plant lignocellulose engineering.Plant biotechnology (Tokyo, Japan) · 2024Article
- Expression of dehydroshikimate dehydratase in poplar induces transcriptional and metabolic changes in the phenylpropanoid pathway.Journal of experimental botany · 2024Article
- Enhancing monolignol ferulate conjugate levels in poplar lignin via OsFMT1.Biotechnology for biofuels and bioproducts · 2024Article
- Genome-wide identification of tea plant (Camellia sinensis) BAHD acyltransferases reveals their role in response to herbivorous pests.BMC plant biology · 2024Article
- Genome-Wide Identification and Expression Pattern Analysis of BAHD Acyltransferase Family inInternational journal of molecular sciences · 2024Article
- Lignin Hydrogenolysis: Phenolic Monomers from Lignin and Associated Phenolates across Plant Clades.ACS sustainable chemistry & engineering · 2023Article
- Rapid Biocatalytic Synthesis of Aromatic Acid CoA Thioesters by Using Microbial Aromatic Acid CoA Ligases.Chembiochem : a European journal of chemical biology · 2023Article
- Metabolic engineering of p-hydroxybenzoate in poplar lignin.Plant biotechnology journal · 2023Article
- Novel candidate genes for lignin structure identified through genome-wide association study of naturally varyingFrontiers in plant science · 2023Article
- Molecular simulation of lignin-related aromatic compound permeation through gram-negative bacterial outer membranes.The Journal of biological chemistry · 2022Article
- Engineering sorghum for higher 4-hydroxybenzoic acid content.Metabolic engineering communications · 2022Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- A new approach to zip-lignin: 3,4-dihydroxybenzoate is compatible with lignification.The New phytologist · 2022Article
- Engineering Curcumin Biosynthesis in Poplar Affects Lignification and Biomass Yield.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poplar (Populus) lignin is naturally acylated with p-hydroxybenzoate ester moieties. However, the enzyme(s) involved in the biosynthesis of the monolignol-p-hydroxybenzoates have remained largely unknown. Here, we performed an in vitro screen of the Populus trichocarpa BAHD acyltransferase superfamily (116 genes) using a wheatgerm cell-free translation system and found five enzymes capable of producing monolignol-p-hydroxybenzoates. We then compared the transcript abundance of the five corresponding genes with p-hydroxybenzoate concentrations using naturally occurring unrelated genotypes of P. trichocarpa and revealed a positive correlation between the expression of p-hydroxybenzoyl-CoA monolig-nol transferase (pHBMT1, Potri.001G448000) and p-hydroxybenzoate levels. To test whether pHBMT1 is responsible for the biosynthesis of monolignol-p-hydroxybenzoates, we overexpressed pHBMT1 in hybrid poplar (Populus alba × P. grandidentata) (35S::pHBMT1 and C4H::pHBMT1). Using three complementary analytical methods, we showed that there was an increase in soluble monolignol-p-hydroxybenzoates and cell-wall-bound monolignol-p-hydroxybenzoates in the poplar transgenics. As these pendent groups are ester-linked, saponification releases p-hydroxybenzoate, a precursor to parabens that are used in pharmaceuticals and cosmetics. This identified gene could therefore be used to engineer lignocellulosic biomass with increased value for emerging biorefinery strategies.
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