Purification and preparation of

Daniel Jun, Sylvester Zhang, Adrian Jan Grzędowski, Amita Mahey, J Thomas Beatty, Dan Bizzotto

Open access · goldAbstract read

In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 87% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Daniel JunAdvanced Materials and Process Engineering Laboratory, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

Sylvester ZhangAdvanced Materials and Process Engineering Laboratory, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

Adrian Jan GrzędowskiAdvanced Materials and Process Engineering Laboratory, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

Amita MaheyDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z1, Canada.

J Thomas BeattyDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z1, Canada.

Dan BizzottoAdvanced Materials and Process Engineering Laboratory, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

University of British Columbia · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The formation of defined surfaces consisting of photosynthetic reaction centers (RCs) in biohybrid solar cells is challenging. Here, we start with the production of engineered RCs for oriented binding. RCs are deposited onto gold electrodes, and 6-mercapto-1-hexanol (MCH) is used to displace multilayers and non-specifically adsorbed RCs. The resulting electrode surfaces are analyzed for photocurrent generation using an intensity-modulated light and lock-in amplifier. Atomic force microscopy (AFM) is used to characterize the surface and the formation of RC structural assemblies. For complete details on the use and execution of this profile, please refer to Jun et al. (2021).

Indexed as

Photosynthetic Reaction Center Complex ProteinsRhodobacter sphaeroidesElectrodesGoldMicroscopy, Atomic ForceGoldPhotosynthetic Reaction Center Complex ProteinsAtomic Force Microscopy (AFM)BiophysicsChemistryEnergyMaterial sciencesMicrobiologyMicroscopyPhysicsProtein BiochemistryProtein expression and purification

Identifiers

PMID34977685

DOI10.1016/j.xpro.2021.101044

PMCPMC8689352

OpenAlexW4200223906

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.