Evidence map›Paper›PMID 34977598›Full record

ArticleCurrent research in structural biology2022

Super-resolution confocal cryo-CLEM with cryo-FIB milling for

Danielle L Sexton, Steffen Burgold, Andreas Schertel, Elitza I Tocheva

Open access · goldAbstract read
In one paragraph

Article in Current research in structural biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
8.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 63 citations in OpenAlex.

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  11. Combining live fluorescence imaging with in situ cryoelectron tomography sheds light on the septation process inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  12. Review
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  14. Liquid Phase Electron Microscopy of Bacterial Ultrastructure.Small (Weinheim an der Bergstrasse, Germany) · 2024
    Article
  15. Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024
    Review
  16. Review
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  20. The cell envelope architecture of Deinococcus: HPI forms the S-layer and SlpA tethers the outer membrane to peptidoglycan.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Danielle L SextonDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
Steffen BurgoldZEISS Microscopy Customer Center, Oberkochen, Germany.
Andreas SchertelZEISS Microscopy Customer Center, Oberkochen, Germany.
Elitza I TochevaDepartment of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
Carl Zeiss (Germany) · DEUniversity of British Columbia · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Studying bacterial cell envelope architecture with electron microscopy is challenging due to the poor preservation of microbial ultrastructure with traditional methods. Here, we established and validated a super-resolution cryo-correlative light and electron microscopy (cryo-CLEM) method, and combined it with cryo-focused ion beam (cryo-FIB) milling and scanning electron microscopy (SEM) volume imaging to structurally characterize the bacterium

Indexed as

Cell envelope architectureCryo-CLEMCryo-ETCryo-FIBCryo-super resolution microscopyMicrobial ultrastructure

Identifiers

PMID34977598
PMCPMC8688812
OpenAlexW4200104884

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.