ArticleFrontiers in cell and developmental biology2021
Assessing Phototoxicity in a Mammalian Cell Line: How Low Levels of Blue Light Affect Motility in PC3 Cells.
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Remote-Controlled Release of Nitric Oxide by Plasmonic Waveguide Coated With Metal-Organic Frameworks.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Identifying lipid-protein interactomes with functional lipid probes.Nature protocols · 2026Review
- Photobiomodulation of tissue regeneration: low-power light therapy accelerates wound healing in earthworms.Biologia futura · 2026Article
- Anticipation of periodic events influences cell motility in amoeba proteus.Scientific reports · 2026Article
- An Integrated Method for Profiling Lipid-Protein Interactions Using Multifunctional Lipid Probes.bioRxiv : the preprint server for biology · 2026Article
- The photophobic response in the apusomonad Podomonas kaiyoae is mediated by coordination of cilia and actin filaments.Communications biology · 2025Article
- PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.Nature communications · 2025Article
- Noninvasive real-time monitoring of cellular spatiotemporal dynamics via machine learning-enhanced electrical impedance spectroscopy.Science advances · 2025Article
- High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization.Scientific reports · 2025Article
- The Curse of the Red Pearl: A Fibroblast-Specific Pearl-Necklace Mitochondrial Phenotype Caused by Phototoxicity.Biomolecules · 2025Article
- Label-Free Prediction of Fluorescently Labeled Fibrin Networks.Biomaterials research · 2025Article
- Effect of combined blue light and 5-ALA on mitochondrial functions and cellular responses in B16F1 melanoma and HaCaT cells.Cytotechnology · 2024Article
- Optogenetically engineered Septin-7 enhances immune cell infiltration of tumor spheroids.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization.View (Beijing, China) · 2024Article
- The effect of continuous long-term illumination with visible light in different spectral ranges on mammalian cells.Scientific reports · 2024Article
- Harnessing artificial intelligence to reduce phototoxicity in live imaging.Journal of cell science · 2024Article
- Potential consequences of phototoxicity on cell function during live imaging of intestinal organoids.PloS one · 2024Article
- High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies.PloS one · 2024Article
- Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study.Communications biology · 2023Article
- An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells.Nature communications · 2023Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phototoxicity is a significant constraint for live cell fluorescence microscopy. Excessive excitation light intensities change the homeostasis of the observed cells. Erroneous and misleading conclusions may be the problematic consequence of observing such light-induced pathophysiology. In this study, we assess the effect of blue light, as commonly used for GFP and YFP excitation, on a motile mammalian cell line. Tracking PC3 cells at different light doses and intensities, we show how motility can be used to reliably assess subtle positive and negative effects of illumination. We further show that the effects are a factor of intensity rather than light dose. Mitotic delay was not a sensitive indicator of phototoxicity. For early detection of the effect of blue light, we analysed the expression of genes involved in oxidative stress. This study addresses the need for relatively simple and sensitive methods to establish a dose-response curve for phototoxicity in mammalian cell line models. We conclude with a working model for phototoxicity and recommendations for its assessment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.