Evidence map›Paper›PMID 34976983›Full record

ArticleFrontiers in bioengineering and biotechnology2021

Using Unnatural Protein Fusions to Engineer a Coenzyme Self-Sufficiency System for D-Phenyllactic Acid Biosynthesis in

Zhao Qin, Dan Wang, Ruoshi Luo, Tinglan Li, Xiaochao Xiong, Peng Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. SERS-based microdroplet platform for high-throughput screening ofFrontiers in bioengineering and biotechnology · 2024
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Zhao QinSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.
Dan WangSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.
Ruoshi LuoSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.
Tinglan LiSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.
Xiaochao XiongDepartment of Biological Systems Engineering, Washington State University, Pullman, WA, United States.
Peng ChenSchool of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.
Chongqing University · CNWashington State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The biosynthetic production of D-penyllactic acid (D-PLA) is often affected by insufficient supply and regeneration of cofactors, leading to high production cost, and difficulty in industrialization. In this study, a D-lactate dehydrogenase (D-LDH) and glycerol dehydrogenase (GlyDH) co-expression system was constructed to achieve coenzyme NADH self-sufficiency and sustainable production of D-PLA. Using glycerol and sodium phenylpyruvate (PPA) as co-substrate, the

Indexed as

coenzyme self-sufficiencyd-Lactate dehydrogenaseD-PLAfusion proteinglycerol dehydrogenasephenyllactic acid

Identifiers

PMID34976983
PMCPMC8718758
OpenAlexW4200312757

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.