Evidence map›Paper›PMID 34973965›Full record

ArticleExperimental neurology2022

Harnessing rAAV-retro for gene manipulations in multiple pathways that are interrupted after spinal cord injury.

Mariajose Metcalfe, Kelly M Yee, Juan Luo, Jacob H Martin-Thompson, Sunil P Gandhi, Oswald Steward

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental neurology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Nonresolving Neuroinflammation Regulates Axon Regeneration in Chronic Spinal Cord Injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025
    Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Mariajose MetcalfeReeve-Irvine Research Center, University of California Irvine School of Medicine, USA; Department of Anatomy & Neurobiology, University of California Irvine School of Medicine, USA.
Kelly M YeeReeve-Irvine Research Center, University of California Irvine School of Medicine, USA; Department of Anatomy & Neurobiology, University of California Irvine School of Medicine, USA.
Juan LuoDepartment of Physiology, Tongji Medical College, Huazhong University of Science and Technology, China.
Jacob H Martin-ThompsonDepartment of Neurobiology & Behavior, University of California Irvine, USA.
Sunil P GandhiDepartment of Neurobiology & Behavior, University of California Irvine, USA.
Oswald StewardReeve-Irvine Research Center, University of California Irvine School of Medicine, USA; Department of Anatomy & Neurobiology, University of California Irvine School of Medicine, USA; Department of Neurobiology & Behavior, University of California Irvine, USA; Department of Neurosurgery, University of California Irvine School of Medicine, USA. Electronic address: osteward@uci.edu.
University of California, Irvine · USHuazhong University of Science and Technology · CN

Funding

Mechanisms of Corticospinal Tract RegenerationR01NS108189 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI STEWARD, OSWALD · 2019 to 2022
$2.3M
NINDS NIH HHS R01 NS108189
6 · The paper itself

Abstract

This paper explores the potential of rAAV2-retro to deliver gene modifying cargoes to the cells of origin of multiple pathways that are interrupted by spinal cord injury (SCI), summarizing data from previous studies and new data from additional experiments. rAAV-retro exhibits uniquely robust and reliable long-distance retrograde transport from pre-terminal axons and synapses back to neuronal bodies. Previous studies have documented that various AAV-based genetic modifications can enable axon regeneration after SCI, but these have targeted the cells of origin of one pathway at a time. In contrast, rAAV-retro can simultaneously transduce large numbers of neurons of origin of multiple spinal pathways with single injections into the spinal cord. Our initial studies use Rosa

Indexed as

AnimalsAxonsFemaleGenetic TherapyGenetic VectorsHumansMaleMiceMice, KnockoutMice, TransgenicNerve RegenerationNeural PathwaysPTEN PhosphohydrolaseRatsRats, Sprague-DawleyRNA, Small InterferingPTEN PhosphohydrolasePten protein, ratRNA, Small InterferingAAV-based gene modificationCorticospinal tractMouserAAV-retroRatRetrograde transportSpinal cordSpinal pathways

Identifiers

PMID34973965
PMCPMC9055973
OpenAlexW4200171636

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.