Evidence map›Paper›PMID 34972942›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis.

Debadrita Pal, Florencia Visconti, Silvia P Sepúlveda-Ramírez, S Zachary Swartz, Charles B Shuster

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Debadrita PalDepartment of Biology, New Mexico State University, Las Cruces, NM, USA.
Florencia ViscontiDepartment of Biology, New Mexico State University, Las Cruces, NM, USA.
Silvia P Sepúlveda-RamírezDepartment of Biology, New Mexico State University, Las Cruces, NM, USA.
S Zachary SwartzWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Charles B ShusterDepartment of Biology, New Mexico State University, Las Cruces, NM, USA. cshuster@nmsu.edu.
New Mexico State University · USWhitehead Institute for Biomedical Research · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The early embryos of sea urchins and other echinoderms have served as experimental models for the study of cell division since the nineteenth century. Their rapid development, optical clarity, and ease of manipulation continue to offer advantages for studying spindle assembly and cytokinesis. In the absence of transgenic lines, alternative strategies must be employed to visualize microtubules and actin. Here, we describe methods to visualize actin and microtubule using either purified, recombinant proteins, or probes in in vitro-transcribed mRNAs.

Indexed as

MicrotubulesMitosisAnimalsGerm CellsMeiosisSea UrchinsSpindle ApparatusConfocal microscopyCytokinesis actinMeiosisMicrotubuleMitosisSea starSea urchin

Identifiers

PMID34972942
OpenAlexW4205890668

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.