Evidence map›Paper›PMID 34972929›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding Proteins Including Natural Anti-Glycan Antibodies.

Sharad Purohit, Jin-Xiong She

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Serum antibody screening using glycan arrays.Chemical Society reviews · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Sharad PurohitCenter for Biotechnology and Genomic Medicine, Augusta University, Augusta, GA, USA.
Jin-Xiong SheCenter for Biotechnology and Genomic Medicine, Augusta University, Augusta, GA, USA. jshe@augusta.edu.

Funding

Luminex multiplex glycan array (LMGA): a high throughput and high content platform for GBPsU01CA221242 · NCI · AUGUSTA UNIVERSITY · PI SHE, JIN-XIONG · 2017 to 2019
$1.4M
Multiplex Luminex glycan array for large scale analyses of glycan-binding proteinsR21CA199868 · NCI · AUGUSTA UNIVERSITY · PI SHE, JIN-XIONG · 2015 to 2016
$526k
NCI NIH HHS R21 CA199868NCI NIH HHS U01 CA221242
6 · The paper itself

Abstract

We present here detailed protocols for the newly developed multiplex glycan bead array (MGBA) for the high throughput and high content analyses of various glycan-binding proteins including anti-glycan antibodies. This platform takes advantage of the commercially available Luminex beads to construct glycan arrays that are easily customizable at will and anytime by researchers. The platform allows the simultaneous analyses of up to 500 glycans and 384 samples at a time. By using multiple arrays, a researcher can analyze thousands of glycans and tens of thousands of samples within a short period. The assay is highly sensitive, specific, reproducible, economic, and fast. Furthermore, the bead array platform is approved for use in clinical settings, speeding up the translation of laboratory discoveries into patient care.

Indexed as

Carrier ProteinsPolysaccharidesAntibodiesHumansMitoguazoneProteinsAntibodiesCarrier Proteinsmethylglyoxal bis(3-aminopropylamidinohydrazone)MitoguazonePolysaccharidesProteinsArraysBeadsBiomarkersCarbohydratesGlycan antibodiesGlycansGlycoconjugatesLuminexPolysaccharides

Identifiers

PMID34972929
PMCPMC9284344

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.