ArticlePloS one2021
Effects of osmolality and solutes on the morphology of red blood cells according to three-dimensional refractive index tomography.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Impact of Different Red Blood Cell Storage Solutions and Conditions on Cell Function and Viability: A Systematic Review.Biomolecules · 2024Pooled it
- Radiation exposure of urologists during endourological procedures: a systematic review.World journal of urology · 2024Pooled it
- Article
- Programming Degradation and Drug Release Through Micropatterning of PLGA Films.ACS applied materials & interfaces · 2026Article
- Seeing What Sticks: Anchoring Capabilities of Moieties for Use in Cell-Conveyed Therapeutics.Bioconjugate chemistry · 2025Article
- The Mechanical Properties of Erythrocytes Are Influenced by the Conformational State of Albumin.Cells · 2025Review
- 3D quantitative phase imagingRSC advances · 2025Article
- Cytocompatibility Study of Stainless Steel 316l Against Differentiated SH-SY5Y Cells.Biomimetics (Basel, Switzerland) · 2025Article
- Cold storage surpasses the impact of biological age and donor characteristics on red blood cell morphology classified by deep machine learning.Scientific reports · 2025Article
- Oxidative impact on lipoprotein structure: Insights from dynamic light scattering.Biochemistry and biophysics reports · 2025Article
- Donor age contributes more to the rheological properties of stored red blood cells than donor sex and biological age distribution.Blood advances · 2025Article
- Validation of direct recording of electrically evoked cortical auditory evoked potentials through a cochlear implant system.Scientific reports · 2024Article
- Label-Free Visualization and Morphological Profiling of Neuronal Differentiation and Axonal Degeneration through Quantitative Phase Imaging.Advanced biology · 2024Article
- Optimization of Formaldehyde Fixative Concentration for Individual Blood Cells to Develop a Stabilized Blood Control for Automated Hematology Analyzers.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2024Article
- Swelling Behavior of Novel Hydrogels Produced from Glucose-Based Ionic Monomers with Varying Cross-Linkers.ACS omega · 2024Article
- [Current CT developments in imaging of pancreatic diseases].Radiologie (Heidelberg, Germany) · 2023Review
- Quantitative Measurements of Red Blood Cell Indices Using Spectroscopic Differential Phase-Contrast Microscopy.Chemical & biomedical imaging · 2023Article
- Article
- Article
- Effects of Coincubation With Crystalloids or Medications on Canine Packed Red Blood Cells: An In Vitro Evaluation.Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphate-buffered saline (PBS) and Alsever's solution (AS) are frequently used as media in blood-related studies, while 0.9% normal saline (NS) is frequently used in transfusion medicine. Despite the frequent use, the effects of these solutions on the shape and volume of red blood cells (RBCs) have not been reported. We collected blood samples from five healthy adults and used three-dimensional refractive index tomography to investigate the changes in the morphology of RBCs caused by changes in osmolality and solutes at the single-cell level. After diluting 2 μL of RBCs 200-fold with each solution (PBS, AS, and 0.9% NS), 40 randomly selected RBCs were microscopically observed. RBC shape was measured considering sphericity, which is a dimensionless quantity ranging from 0 (flat) to 1 (spherical). RBCs in plasma or AS showed a biconcave shape with a small sphericity, whereas those in 0.9% NS or PBS showed a spherical shape with a large sphericity. Moreover, we confirmed that sodium chloride alone could not elicit the biconcave shape of RBCs, which could be maintained only in the presence of an osmotic pressure-maintaining substance, such as glucose or mannitol. Although 0.9% NS solution is one of the most commonly used fluids in hematology and transfusion medicine, RBCs in 0.9% NS or PBS are not biconcave. Therefore, as the debate on the use of NS continues, future clinical studies or applications should consider the effect of glucose or mannitol on the shape of RBCs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.