ArticleScience China. Life sciences2022
Exogenous artificial DNA forms chromatin structure with active transcription in yeast.
Article in Science China. Life sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 20 citations in OpenAlex.
- ExoShorkie: predicting RNA-seq coverage of exogenous genomes in yeast by transfer learning.Bioinformatics (Oxford, England) · 2026Article
- Pervasive Transcription in the Human Genome Exceeds Background Noise.Genome biology and evolution · 2026Article
- Automated linear DNA assembly of A. thaliana's chloroplast and mitochondrial genome.Nucleic acids research · 2026Article
- Cellular lipids: fundamental host factors for monkeypox virus replication and pathogenesis.Archives of microbiology · 2026Review
- Intergeneric chromosomal transfer in yeast results in improved phenotypes and widespread transcriptional responses.Nature communications · 2026Article
- Design and structure of overlapping regions in PCA via deep learning.Synthetic and systems biotechnology · 2025Article
- Pragmatic soft-decision data readout of encoded large DNA.Briefings in bioinformatics · 2025Article
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- Regulatory activity is the default DNA state in eukaryotes.Nature structural & molecular biology · 2024Article
- Machine learning-aided scoring of synthesis difficulties for designer chromosomes.Science China. Life sciences · 2023Article
- SS-31 Improves Cognitive Function in Sepsis-Associated Encephalopathy by Inhibiting the Drp1-NLRP3 Inflammasome Activation.Neuromolecular medicine · 2023Article
- DNA Data Storage.Biotech (Basel (Switzerland)) · 2023Review
- Mobile and Self-Sustained Data Storage in an Extremophile Genomic DNA.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- The Synthesis and Assembly of a Truncated Cyanophage Genome and Its Expression in a Heterogenous Host.Life (Basel, Switzerland) · 2022Article
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12 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Yeast artificial chromosomes (YACs) are important tools for sequencing, gene cloning, and transferring large quantities of genetic information. However, the structure and activity of YAC chromatin, as well as the unintended impacts of introducing foreign DNA sequences on DNA-associated biochemical events, have not been widely explored. Here, we showed that abundant genetic elements like TATA box and transcription factor-binding motifs occurred unintentionally in a previously reported data-carrying chromosome (dChr). In addition, we used state-of-the-art sequencing technologies to comprehensively profile the genetic, epigenetic, transcriptional, and proteomic characteristics of the exogenous dChr. We found that the data-carrying DNA formed active chromatin with high chromatin accessibility and H3K4 tri-methylation levels. The dChr also displayed highly pervasive transcriptional ability and transcribed hundreds of noncoding RNAs. The results demonstrated that exogenous artificial chromosomes formed chromatin structures and did not remain as naked or loose plasmids. A better understanding of the YAC chromatin nature will improve our ability to design better data-storage chromosomes.
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