Evidence map›Paper›PMID 34970254›Full record

ReviewFrontiers in immunology2021

A Recent Update on Advanced Molecular Diagnostic Techniques for COVID-19 Pandemic: An Overview.

Akanksha Roberts, Raghuraj Singh Chouhan, Deepshikha Shahdeo, Narlawar Sagar Shrikrishna, Veerbhan Kesarwani, Milena Horvat, Sonu Gandhi

Open access · goldAbstract readReview
In one paragraph

Review in Frontiers in immunology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
3.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 72 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Akanksha RobertsDepartment of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
Raghuraj Singh ChouhanDepartment of Environmental Sciences, Jožef Stefan Institute, Ljubljana, Slovenia.
Deepshikha ShahdeoDepartment of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
Narlawar Sagar ShrikrishnaDepartment of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
Veerbhan KesarwaniDepartment of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
Milena HorvatDepartment of Environmental Sciences, Jožef Stefan Institute, Ljubljana, Slovenia.
Sonu GandhiDepartment of Biotechnology (DBT)-National Institute of Animal Biotechnology (NIAB), Hyderabad, India.
National Institute of Animal Biotechnology · INJožef Stefan Institute · SI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Coronavirus disease 2019 (COVID-19), which started out as an outbreak of pneumonia, has now turned into a pandemic due to its rapid transmission. Besides developing a vaccine, rapid, accurate, and cost-effective diagnosis is essential for monitoring and combating the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its related variants on time with precision and accuracy. Currently, the gold standard for detection of SARS-CoV-2 is Reverse Transcription Polymerase Chain Reaction (RT-PCR), but it lacks accuracy, is time-consuming and cumbersome, and fails to detect multi-variant forms of the virus. Herein, we have summarized conventional diagnostic methods such as Chest-CT (Computed Tomography), RT-PCR, Loop Mediated Isothermal Amplification (LAMP), Reverse Transcription-LAMP (RT-LAMP), as well new modern diagnostics such as CRISPR-Cas-based assays, Surface Enhanced Raman Spectroscopy (SERS), Lateral Flow Assays (LFA), Graphene-Field Effect Transistor (GraFET), electrochemical sensors, immunosensors, antisense oligonucleotides (ASOs)-based assays, and microarrays for SARS-CoV-2 detection. This review will also provide an insight into an ongoing research and the possibility of developing more economical tools to tackle the COVID-19 pandemic.

Indexed as

Clinical Laboratory TechniquesCOVID-19COVID-19 TestingHumansImmunoassayMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesOligonucleotide ProbesPandemicsReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA, ViralSARS-CoV-2Sensitivity and SpecificityOligonucleotide ProbesRNA, ViralbiosensorsCRISPR-CasdiagnosticsLAMPpoint of careRT-PCRSARS-CoV-2

Identifiers

PMID34970254
PMCPMC8712736
OpenAlexW4200003748

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.