ArticleNucleic acids research2022
Nonalternating purine pyrimidine sequences can form stable left-handed DNA duplex by strong topological constraint.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed.
- Biological consequences of Z-nucleic acid sensing by ZBP1 and ADAR1.RNA biology · 2026Review
- Preparation of topoisomers of short circular dsDNA with defined linking number by accurate topological control.Nucleic acids research · 2025Article
- Computational Simulation and Biophysical Study on Cerium Chloride-Induced B-to-Z Transition in (CG)ACS omega · 2024Article
- Assessing B-Z DNA Transitions in Solutions via Infrared Spectroscopy.Biomolecules · 2023Article
- A fish herpesvirus highlights functional diversities among Zα domains related to phase separation induction and A-to-Z conversion.Nucleic acids research · 2023Article
- Construction of ssDNA-Attached LR-Chimera Involving Z-DNA for ZBP1 Binding Analysis.Molecules (Basel, Switzerland) · 2022Article
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8 authors.
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Abstract
In vivo, left-handed DNA duplex (usually refers to Z-DNA) is mainly formed in the region of DNA with alternating purine pyrimidine (APP) sequence and plays significant biological roles. It is well known that d(CG)n sequence can form Z-DNA most easily under negative supercoil conditions, but its essence has not been well clarified. The study on sequence dependence of Z-DNA stability is very difficult without modification or inducers. Here, by the strong topological constraint caused by hybridization of two complementary short circular ssDNAs, left-handed duplex part was generated for various sequences, and their characteristics were investigated by using gel-shift after binding to specific proteins, CD and Tm analysis, and restriction enzyme cleavage. Under the strong topological constraint, non-APP sequences can also form left-handed DNA duplex as stable as that of APP sequences. As compared with non-APP sequences, the thermal stability difference for APP sequences between Z-form and B-form is smaller, which may be the reason that Z-DNA forms preferentially for APP ones. This result can help us to understand why nature selected APP sequences to regulate gene expression by transient Z-DNA formation, as well as why polymer with chirality can usually form both duplexes with left- or right-handed helix.
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