Evidence map›Paper›PMID 34966174›Full record

ArticleScientific reports2021

Antimicrobial and cytotoxic activity of electrosprayed chitosan nanoparticles against endodontic pathogens and Balb/c 3T3 fibroblast cells.

Amir Ibrahim, Desigar Moodley, Cosmas Uche, Ernest Maboza, Annette Olivier, Leslie Petrik

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Polymeric nanoparticles delivery circumvents bacterial resistance to ciprofloxacin.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2024
    Article
  7. Review
  8. Review
  9. Therapeutic applications of nanobiotechnology.Journal of nanobiotechnology · 2023
    Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Amir IbrahimDepartment of Restorative Dental Sciences, Faculty of Dentistry, Nile University, Khartoum North, Khartoum, Sudan. dr.aio.ibrahim@gmail.com.
Desigar MoodleyDepartment of Restorative Dentistry, Faculty of Dentistry, University of the Western Cape, Cape Town, South Africa.
Cosmas UcheDepartment of Chemistry, Faculty of Natural Sciences, University of the Western Cape, Cape Town, South Africa.
Ernest MabozaOral and Dental Research Laboratory, Faculty of Dentistry, University of the Western Cape, Cape Town, South Africa.
Annette OlivierOral and Dental Research Laboratory, Faculty of Dentistry, University of the Western Cape, Cape Town, South Africa.
Leslie PetrikDepartment of Chemistry, Faculty of Natural Sciences, University of the Western Cape, Cape Town, South Africa.
University of the Western Cape · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aims of this study were to synthesize highly positively charged chitosan nanoparticles (Ch-Np) using the electrospraying technique, and to test their antimicrobial activity against endodontic pathogens, and cytotoxicity against fibroblast cells. Ch-Np were synthesized from low molecular weight chitosan (LMW-Ch) using the electrospraying technique, and characterized. The antimicrobial activity was evaluated against Streptococcus mutans, Enterococcus faecalis, and Candida albicans in their planktonic state using a Time-Kill Test performed by using broth micro-dilution technique, and against biofilm biomass using a microtiter plate biofilm assay. The cytotoxicity was evaluated using Balb/c 3T3 fibroblast cells with the standard MTT assay. Electrospraying of LMW-Ch produced Ch-Np with an average size of 200 nm, and a surface charge of 51.7 mV. Ch-Np completely eradicated S. mutans and E. faecalis in the planktonic state and showed fungistatic activity against C. albicans. Furthermore, it significantly reduced the biofilm biomass for all the tested microbial species [S. mutans (p = 0.006), E. faecalis (p < 0.0001), and C. albicans (p = 0.004)]. When tested for cytotoxicity using 3T3 cells, Ch-Np showed no cytotoxicity. In conclusion, the highly positively charged, colloidal dispersion of Ch-Np are effective as a biocompatible endodontic antimicrobial agent.

Indexed as

NanoparticlesAnimalsAnti-Infective AgentsBALB 3T3 CellsCandida albicansCell SurvivalChitosanEnterococcus faecalisFibroblastsMiceStreptococcus mutansAnti-Infective AgentsChitosan

Identifiers

PMID34966174
PMCPMC8716534
OpenAlexW4200165666

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.